Abstract
Major components of the brain Renin‐Angiotensin System may provide modes of therapy for impaired neurons. Angiotensin II via its receptors, AT1 and AT2, induces proliferation and differentiation of rodent neural stem cells. This study was undertaken to determine if AT1 and AT2 receptor stimulation by Angiotensin II induces proliferation and differentiation of human neural stem cells (hNSC). This study will assess whether selective agonism of AT1 and AT2 receptors on hNSC could possibly induce proliferation and differentiation in damaged areas of the brain caused by stroke, traumatic brain injury, and several neurodegenerative diseases in a therapeutic manner. Using hNSC, this study aims to enhance our understanding of how neural differentiation and proliferation are affected by selective agonism of both the AT1 and AT2 receptor. It was hypothesized that stimulating the AT1 receptor would induce proliferation and stimulating the AT2 receptor would induce differentiation upon addition of selective agonists. Preliminary analyses of this ongoing research support the hypothesis, as there was a 78% increase in neuronal differentiation induced by AT2 agonism, and a 5% increase in proliferation with selective AT1 agonism. Further investigation of these preliminary results will determine if these trends are genuine and statistically significant.
Faculty Sponsors
James Munoz, Ph.D., Mark Jaffe, D.P.M., Robert Speth, Ph.D.
Project Type
Event
Location
Alvin Sherman Library
Start Date
4-10-2015 1:00 PM
End Date
4-10-2015 5:30 PM
Neural Stem Cell Proliferation, Differentiation, and Survival in Response to AT1 and AT2 Angiotensin II Receptor-Specific Agonism
Alvin Sherman Library
Major components of the brain Renin‐Angiotensin System may provide modes of therapy for impaired neurons. Angiotensin II via its receptors, AT1 and AT2, induces proliferation and differentiation of rodent neural stem cells. This study was undertaken to determine if AT1 and AT2 receptor stimulation by Angiotensin II induces proliferation and differentiation of human neural stem cells (hNSC). This study will assess whether selective agonism of AT1 and AT2 receptors on hNSC could possibly induce proliferation and differentiation in damaged areas of the brain caused by stroke, traumatic brain injury, and several neurodegenerative diseases in a therapeutic manner. Using hNSC, this study aims to enhance our understanding of how neural differentiation and proliferation are affected by selective agonism of both the AT1 and AT2 receptor. It was hypothesized that stimulating the AT1 receptor would induce proliferation and stimulating the AT2 receptor would induce differentiation upon addition of selective agonists. Preliminary analyses of this ongoing research support the hypothesis, as there was a 78% increase in neuronal differentiation induced by AT2 agonism, and a 5% increase in proliferation with selective AT1 agonism. Further investigation of these preliminary results will determine if these trends are genuine and statistically significant.
