Abstract
Flavone A and B were found to be cytotoxic in pancreatic and colon cancers. Both work in triggering cell apoptosis, although the mechanism and relationship between structure and function are unknown. They are similar in structure, only differing in the location of a hydroxyl and methoxyl group. Flavone B is more planar and lipophilic and, in contrast to Flavone A, has been found to selectively induce apoptosis in less differentiated cancer cells. In this study, the goal was to trace flavone uptake by pancreatic cancer cells and its dispersion inside to determine whether apoptosis is caused in the nucleus or cytoplasm. Two pancreatic cancer cell types were used, PANC28 and MiaPaca, of which PANC28 is more differentiated. It is known that the cytotoxic effects are limited to Flavone A in PANC28 and Flavone B in MiaPaca. Samples of both cell types were dosed with a 40 μM concentration of Flavone A and B in separate trials with exposure times of 1, 10, and 30 minutes with different oexposure times for the cytoplasmic and nuclear fractions obtained through their separation. The fractions were analyzed using GCMS with a Rxi-5ms column. Flavone A appeared in both nuclear and cytoplasmic fractions of Panc28 and MiaPaca. Flavone B appeared only in the nuclear fraction of Panc28 and the cytoplasmic fraction of MiaPaca. This suggests that Flavone A localizes in cytoplasm but interacts with nuclear components to trigger apoptosis, while Flavone B induces death of the MiaPaca cancer cells from its location in the cytoplasm.
Faculty Sponsors
Dr. Maria Ballester
Project Type
Event
Location
Alvin Sherman Library
Start Date
4-4-2014 1:00 PM
End Date
4-4-2014 5:30 PM
Analysis by GCMS of Flavone Isomers in Pancreatic Cancer Cells
Alvin Sherman Library
Flavone A and B were found to be cytotoxic in pancreatic and colon cancers. Both work in triggering cell apoptosis, although the mechanism and relationship between structure and function are unknown. They are similar in structure, only differing in the location of a hydroxyl and methoxyl group. Flavone B is more planar and lipophilic and, in contrast to Flavone A, has been found to selectively induce apoptosis in less differentiated cancer cells. In this study, the goal was to trace flavone uptake by pancreatic cancer cells and its dispersion inside to determine whether apoptosis is caused in the nucleus or cytoplasm. Two pancreatic cancer cell types were used, PANC28 and MiaPaca, of which PANC28 is more differentiated. It is known that the cytotoxic effects are limited to Flavone A in PANC28 and Flavone B in MiaPaca. Samples of both cell types were dosed with a 40 μM concentration of Flavone A and B in separate trials with exposure times of 1, 10, and 30 minutes with different oexposure times for the cytoplasmic and nuclear fractions obtained through their separation. The fractions were analyzed using GCMS with a Rxi-5ms column. Flavone A appeared in both nuclear and cytoplasmic fractions of Panc28 and MiaPaca. Flavone B appeared only in the nuclear fraction of Panc28 and the cytoplasmic fraction of MiaPaca. This suggests that Flavone A localizes in cytoplasm but interacts with nuclear components to trigger apoptosis, while Flavone B induces death of the MiaPaca cancer cells from its location in the cytoplasm.
