Abstract
Lithocholic acid (LCA), CHO is a bile acid naturally produced in the liver that helps to digest fats. LCA has been reported to produce cytotoxic effects on cancer cells while leaving healthy cells unharmed. Recently, based on previous studies conducted, an apoptotic mechanism was proposed for LCA in neuroblastoma (NB) cells, rat glioma cells and breast cancer cells. Therefore, the main purpose of this experiment was to confirm the apoptosis inducing mechanisms of LCA in LNCaP prostate cancer cells. In addition, a combination of LCA and topotecan (TPT), a chemotherapeutic drug, was used to evaluate its potency as well. The effects of LCA were measured through induction of cytotoxicity, DNA fragmentation, caspase-3 and caspase-9 activity, PARP cleavage, and Western blotting. The results obtained from each experiment confirmed the initial hypothesis of the apoptotic effect of LCA on LNCaP cancer cells. Lithocholic acid alone has shown greater oncolytic potential than the combination tested. (The authors would like to thank Royal Dames of Cancer Research Inc, Ft. Lauderdale)
Faculty Sponsors
Dr. Appu Rathinavelu, Dr. Deanne Roopnarine, Dr. Sivanesan Dhandayuthapani
Project Type
Event
Location
Alvin Sherman Library
Start Date
4-12-2013 1:00 PM
End Date
4-12-2013 5:30 PM
Apoptotic Effect of Lithocholic Acid on LNCaP Prostate Cancer Cell Line
Alvin Sherman Library
Lithocholic acid (LCA), CHO is a bile acid naturally produced in the liver that helps to digest fats. LCA has been reported to produce cytotoxic effects on cancer cells while leaving healthy cells unharmed. Recently, based on previous studies conducted, an apoptotic mechanism was proposed for LCA in neuroblastoma (NB) cells, rat glioma cells and breast cancer cells. Therefore, the main purpose of this experiment was to confirm the apoptosis inducing mechanisms of LCA in LNCaP prostate cancer cells. In addition, a combination of LCA and topotecan (TPT), a chemotherapeutic drug, was used to evaluate its potency as well. The effects of LCA were measured through induction of cytotoxicity, DNA fragmentation, caspase-3 and caspase-9 activity, PARP cleavage, and Western blotting. The results obtained from each experiment confirmed the initial hypothesis of the apoptotic effect of LCA on LNCaP cancer cells. Lithocholic acid alone has shown greater oncolytic potential than the combination tested. (The authors would like to thank Royal Dames of Cancer Research Inc, Ft. Lauderdale)
