Abstract
Breast cancer is a prevalent cancer that occurs in many women and men today. There are many exogenous and endogenous causes of DNA damage as well as a multitude of repair mechanisms to fix the damage; however, if it is not fixed, the damage becomes a permanent mutation. A series of these mutations lead to cancer. Deficient Nucleotide Excision Repair (NER) is being studied as one of the main mechanisms of carcinogenesis. Deficient NER was found in stage I breast cancers as compared to normal breast epithelium and may be due to over-expression of microRNA. In order to verify this process, antagomirs will be transfected into the breast cancer cells in order to knock out certain microRNA expression, then the cells will be irradiated using UV radiation, the samples will then be purified, and the DNA damage will then be detected using ELISA.
If the microRNA expression could be fixed, the gene repair would theoretically increase and the incidence of breast cancer would decrease.
Faculty Sponsors
Dr. Mark Jaffe
Project Type
Event
Location
Alvin Sherman Library
Start Date
4-12-2013 1:00 PM
End Date
4-12-2013 5:30 PM
Nucleotide Excision Repair: A New Component in the Study of Breast Cancer
Alvin Sherman Library
Breast cancer is a prevalent cancer that occurs in many women and men today. There are many exogenous and endogenous causes of DNA damage as well as a multitude of repair mechanisms to fix the damage; however, if it is not fixed, the damage becomes a permanent mutation. A series of these mutations lead to cancer. Deficient Nucleotide Excision Repair (NER) is being studied as one of the main mechanisms of carcinogenesis. Deficient NER was found in stage I breast cancers as compared to normal breast epithelium and may be due to over-expression of microRNA. In order to verify this process, antagomirs will be transfected into the breast cancer cells in order to knock out certain microRNA expression, then the cells will be irradiated using UV radiation, the samples will then be purified, and the DNA damage will then be detected using ELISA.
If the microRNA expression could be fixed, the gene repair would theoretically increase and the incidence of breast cancer would decrease.
