Abstract
The MDM2 oncogene has become one of the major fields of cancer research since this oncogenic protein is expressed in several common human cancers including prostate cancer. MDM2 interacts with many proteins in the cells such as p53 and leads to over expression of PI3K, HIF-1α, VEGF. Hence exploring the role of alterations associated with these genes at the transcription level has become a major part of our prostate cancer research. LNCaP cells that have been transfected (LNCaP-MSI) with a gene silencer specific for MDM2 was speculated to become a good model for some of our advanced studies in prostate cancer. The LNCaP-MSI prostate cancer cells were tested by comparing to normal LNCaP prostate cancer cells and LNCaP-MST (MDM2 transfected) cancer cells to see whether MDM2 silencing had any impact on the expression of PI3K, HIF-1α and VEGF. The results of our experiments with LNCaP-MST cells have offered some valuable information. (This project was supported by Royal dames of Cancer Research Inc, Ft. Lauderdale, Florida)
Faculty Sponsors
Dr. Appu Rathinavelu, Dr. Mark Jaffe
Project Type
Event
Location
Alvin Sherman Library
Start Date
4-12-2013 1:00 PM
End Date
4-12-2013 5:30 PM
Expression Levels of Various Proteins in Wild-type and Transfected LNCaP Prostate Cancer Cells
Alvin Sherman Library
The MDM2 oncogene has become one of the major fields of cancer research since this oncogenic protein is expressed in several common human cancers including prostate cancer. MDM2 interacts with many proteins in the cells such as p53 and leads to over expression of PI3K, HIF-1α, VEGF. Hence exploring the role of alterations associated with these genes at the transcription level has become a major part of our prostate cancer research. LNCaP cells that have been transfected (LNCaP-MSI) with a gene silencer specific for MDM2 was speculated to become a good model for some of our advanced studies in prostate cancer. The LNCaP-MSI prostate cancer cells were tested by comparing to normal LNCaP prostate cancer cells and LNCaP-MST (MDM2 transfected) cancer cells to see whether MDM2 silencing had any impact on the expression of PI3K, HIF-1α and VEGF. The results of our experiments with LNCaP-MST cells have offered some valuable information. (This project was supported by Royal dames of Cancer Research Inc, Ft. Lauderdale, Florida)
