Abstract
Lagenidium giganteum is an entomopathogenic oomycete known for its ability to infect and kill the larvae of mosquitoes. This project seeks to find a potential alternative to chemical insecticides by isolating and sequencing the pathogenic genes found in this organism. More specifically, crinkler genes were targeted, as they have been associated with host cell toxicity in plant pathogens. A combination of several wet-lab techniques such as polymerase chain reaction, gel electrophoresis, gel extraction, and RNA extraction; and the use of bioinformatics tools are used in this project. These techniques will be used for the analysis and screening of the transcriptome for this pathogenic oomycete. The results of this research will not only illustrate the research being done on the crinkler gene, but a secondary pathogenic gene that is also fundamental in this project.
Faculty Sponsors
Dr. Aurelien Tartar
Project Type
Event
Location
Alvin Sherman Library
Start Date
4-13-2012 1:00 PM
End Date
4-13-2012 5:30 PM
The Study of Lagenidium Giganteum Crinkler Genes
Alvin Sherman Library
Lagenidium giganteum is an entomopathogenic oomycete known for its ability to infect and kill the larvae of mosquitoes. This project seeks to find a potential alternative to chemical insecticides by isolating and sequencing the pathogenic genes found in this organism. More specifically, crinkler genes were targeted, as they have been associated with host cell toxicity in plant pathogens. A combination of several wet-lab techniques such as polymerase chain reaction, gel electrophoresis, gel extraction, and RNA extraction; and the use of bioinformatics tools are used in this project. These techniques will be used for the analysis and screening of the transcriptome for this pathogenic oomycete. The results of this research will not only illustrate the research being done on the crinkler gene, but a secondary pathogenic gene that is also fundamental in this project.
