Abstract
One of the most important techniques of research is purification. The objective of the experiment was to collaborate with Veterinarian Scott Hay DVM and Roche Diagnostics to explore the use of purified horse hemoglobin as a model to research glycosylated hemoglobin (HbA1c) in human disease diabetes mellitus types I and II. The international Federation of Clinical Chemistry and Laboratory Medicine have set global standard values for calibrators of HbA1c and the current targets for HbA1c are ~7%. Throughout the experiment horse hemoglobin was isolated by anionic exchange chromatography and glycosylated in-vitro. HbA1c is primarily used for quantification of average plasma glucose concentration. The formation of HbA1c is a non-enzymatic glycosylation called Amadori rearrangement where-in glucose in the horse’s plasma binds to the N-terminal valine residue of the β-chain of hemoglobin in horse red blood cells which forms the irreversible product HbA1c. The HbA1c circulates for the lifespan of the blood cell. Therefore HbA1c is used medically to quantify the day to day blood glucose concentration over the preceding 2-3 months. The rationale for collaborating, monitoring and studying HbA1c is a measure to discover long-term associated risks with researched diabetic complications. The results were analyzed by immunochemical and electrochemical means.
Faculty Sponsors
Dr. Terence McCaffrey
Project Type
Event
Location
Alvin Sherman Library
Start Date
4-13-2012 1:00 PM
End Date
4-13-2012 5:30 PM
The Preparation of Ultrapure Isolated Glycosylated Hemoglobin
Alvin Sherman Library
One of the most important techniques of research is purification. The objective of the experiment was to collaborate with Veterinarian Scott Hay DVM and Roche Diagnostics to explore the use of purified horse hemoglobin as a model to research glycosylated hemoglobin (HbA1c) in human disease diabetes mellitus types I and II. The international Federation of Clinical Chemistry and Laboratory Medicine have set global standard values for calibrators of HbA1c and the current targets for HbA1c are ~7%. Throughout the experiment horse hemoglobin was isolated by anionic exchange chromatography and glycosylated in-vitro. HbA1c is primarily used for quantification of average plasma glucose concentration. The formation of HbA1c is a non-enzymatic glycosylation called Amadori rearrangement where-in glucose in the horse’s plasma binds to the N-terminal valine residue of the β-chain of hemoglobin in horse red blood cells which forms the irreversible product HbA1c. The HbA1c circulates for the lifespan of the blood cell. Therefore HbA1c is used medically to quantify the day to day blood glucose concentration over the preceding 2-3 months. The rationale for collaborating, monitoring and studying HbA1c is a measure to discover long-term associated risks with researched diabetic complications. The results were analyzed by immunochemical and electrochemical means.
