The Study of Lagenidium Giganteum crinkler genes

The Study of Lagenidium Giganteum crinkler genes

Lagenidium giganteum is an entomopathogenic oomycete that is known to infect and kill the larvae of mosquitoes. Due to this characteristic, the organism has been deemed a potential alternative to chemical insecticides. This information is significant when the world issue of mosquito control is considered. The human death toll now reaches one million per year by mosquito-borne diseases, and the research on potential alternatives to chemical insecticides is quite minimal. Only seven L. giganteum protein-coding gene sequences have been reported to date, and the objective of this project is to accelerate the gene research of L. giganteum and identify the genes that are part of the pathogenicity process. In this way, the discovery of a bioinsecticide against mosquitoes could potentially be developed. The study would increase the understanding of the molecular basis interactions of the host and pathogens. The experiment will consists of utilizing the complete genome sequences of currently known six oomycete genomes in order to analyze the complete genome sequence of Lagenidium giganteum. These genes will then be screened for genes that are involved in any pathogenicity process. In this research, polymerase chain reaction and gel electrophoresis techniques will be used for the analysis and screening of the transcriptome for this pathogenic Lagenidium giganteum, and the sequencing of selected full-length transcripts. More specifically, crinkler genes were targeted, as they have been associated with host cell toxicity in plant pathogens.The results of this research will illustrate the progress made in identifying parts of this unique pathogenicity process.