Abstract
Mosquitoes are carriers of numerous diseases for instance, malaria and west-Nile virus. Lagenidium giganteum is a parasitic organism during larval stage that infects and kills mosquitoes. In time, this could be proven to be useful in bioengineering to create an environmentally safe insecticide. Molecular research was performed in order to determine the imperative genes in Lagenidium giganteum that hinder the development in mosquito larvae. A culture of Lagenidium giganteum was used to extract RNA and initiate a transcriptome analysis. Large scale sequencing led to the annotation of 20,000 transcripts. PCR amplification was performed using a Qiagen PCR kit in order to sequence full-length transcripts. Numerous sequences were determined allowing for further analysis of the Lagenidium giganteum genes that are associated with pathogenicity.
Faculty Sponsors
Dr. Aurelien Tartar
Project Type
Event
Start Date
2011 12:00 PM
End Date
2011 5:00 PM
Transcriptome Analysis of Lagenidium giganteum
Mosquitoes are carriers of numerous diseases for instance, malaria and west-Nile virus. Lagenidium giganteum is a parasitic organism during larval stage that infects and kills mosquitoes. In time, this could be proven to be useful in bioengineering to create an environmentally safe insecticide. Molecular research was performed in order to determine the imperative genes in Lagenidium giganteum that hinder the development in mosquito larvae. A culture of Lagenidium giganteum was used to extract RNA and initiate a transcriptome analysis. Large scale sequencing led to the annotation of 20,000 transcripts. PCR amplification was performed using a Qiagen PCR kit in order to sequence full-length transcripts. Numerous sequences were determined allowing for further analysis of the Lagenidium giganteum genes that are associated with pathogenicity.
