Linkage of Restriction Site Polymorphisms at the Minisatellite D1S80 Locus in a Tamil Population from India

Linkage of Restriction Site Polymorphisms at the Minisatellite D1S80 Locus in a Tamil Population from India

In this study, we report the distribution of these polymorphisms in a Tamil population from India. SNP analysis was performed by RT-PCR utilizing a TaqMan® SNP Genotyping Assays kit from Applied Biosystems. Data was collected on a Corbett Rotor- Gene 3000. For most alleles analyzed, the restriction site haplotype polymorphisms were consistent with the assigned older allele types, allele 18 and 24. Appropriate restriction enzymes were used for restricting the PCR products which confirmed all SNP designations. The interesting finding in this study is that 98.5 % (197/200) of the alleles tested have a linkage of two specific polymorphisms. If an allele is positive for Tsp509I, it is positive for Fnu4HI and if it is negative for Tsp509I, then it is negative for Fnu4HI, which shows a tight linkage between the two polymorphic sequences. In most populations studied there is a trimodel apportionment of alleles (Deka, et. al, Hum Genet (1994) 94:252-258). Further study is under way to characterize the sequence and structure of this locus in these samples. Our intent is to infer the age of this SNP and explore the evolution of this SNP and locus in evolutionary time.