Pseudomonas aeruginosa Invades Human Aortic Endothelial Cells and Induces Cell Damage In Vitro

Authors

Rahul Mittal, University of Miami
Vasanti M. Jhaveri, University of Miami
Sae-In Samantha Kay, Nova Southeastern University
Patricia Blackwelder, University of Miami; Nova UniversityFollow
Kunal Patel, University of Miami

Document Type

Article

Publication Title

Cardiovascular & Hematological Disorders-Drug Targets

ISSN

1871-529X

Publication Date

2019

Keywords

Infective endocarditis, Pseudomonas aeruginosa, Cell damage, Cytokines, Lactate dehydrogenase (LDH) assay

Abstract

Background: Cardiovascular diseases such as endocarditis are the second most common cause of death worldwide. Infective Endocarditis (IE) is the most severe infection of the heart associated with significant mortality and morbidity. The binding and invasion of Human Aortic Endothelial Cells (HAECs) by pathogenic microbes can play an important role in the pathogenesis of IE.

Objective: Pseudomonas aeruginosa is an emerging pathogen that has been associated with IE. However, it is not known whether P. aeruginosa can bind and interact with HAECs. The aim of this study was to determine whether P. aeruginosa can bind and colonize HAECs.

Methods: The invasion of HAECs by P. aeruginosa was assessed by gentamicin protection assay. Cytokine levels were determined by enzyme-linked Immunosorbent Assay (ELISA) kits. Cell damage was determined by Lactate Dehydrogenase (LDH) assay.

Results: P. aeruginosa can bind and invade HAECs. Infection of HAECs with P. aeruginosa induces TNF-α IL-1β, IL-6 and IL-8 cytokine production leading to the generation of inflammatory milieu that can cause tissue damage as observed in human clinical cases of IE. We also observed that P. aeruginosa induces cell damage in HAECs.

Conclusion: In this study, we demonstrate for first time that P. aeruginosa can invade and survive inside HAECs. This cell culture model can be of immense importance to determine the efficacy of drug targets against IE.

DOI

10.2174/1871529X18666180611094928

Volume

19

Issue

1

First Page

45

Last Page

50

Comments

©2018 Bentham Science Publishers

NSUWorks Citation

Rahul Mittal, Vasanti M. Jhaveri, Sae-In Samantha Kay, Patricia Blackwelder, and Kunal Patel. 2019. Pseudomonas aeruginosa Invades Human Aortic Endothelial Cells and Induces Cell Damage In Vitro .Cardiovascular & Hematological Disorders-Drug Targets , (1) : 45 -50. https://nsuworks.nova.edu/occ_facarticles/970.