Isolation and Characterization of Polymorphic Microsatellite Markers from a Globally Distributed Marine Apex Predator, the Tiger Shark (Galeocerdo cuvier)

Authors

Andrea Bernard, Nova Southeastern UniversityFollow
Kevin A. Feldheim, Field Museum of Natural History - Chicago
Mahmood S. Shivji, Nova Southeastern UniversityFollow

ResearcherID

G-4080-2013

Document Type

Article

Publication Title

Conservation Genetics Resources

ISSN

1877-7252

Publication Date

6-2015

Keywords

Tiger shark, Galeocerdo cuvier, Microsatellite, Elasmobranch genetics

Abstract

High levels of fishing have resulted in declines among many of the oceans top predators, including the globally distributed tiger shark (Galeocerdo cuvier). Overexploitation of this species has led to declines in parts of its distribution, which may have important ecological consequences given the tiger shark’s trophic position as a large bodied, generalist predator. To assess the population genetics of this species, an enrichment protocol was used to isolate a suite of nine di- and tetra-nucleotide microsatellite loci within the tiger shark, which were subsequently characterized using genomic DNA derived from 40 individuals sampled from Hawaiian waters. The number of alleles per locus ranged from 3 to 23, and the average expected heterozygosities across loci ranged from 0.30 to 0.93. Analyses suggested a low frequency of null alleles across markers, and all loci conformed to both Hardy–Weinberg and linkage equilibrium at P > 0.01.

DOI

10.1007/s12686-014-0408-0

Volume

7

Issue

2

First Page

509

Last Page

511

Comments

©Springer Science+Business Media Dordrecht 2014

NSUWorks Citation

Andrea Bernard, Kevin A. Feldheim, and Mahmood S. Shivji. 2015. Isolation and Characterization of Polymorphic Microsatellite Markers from a Globally Distributed Marine Apex Predator, the Tiger Shark (Galeocerdo cuvier) .Conservation Genetics Resources , (2) : 509 -511. https://nsuworks.nova.edu/occ_facarticles/895.