Marine & Environmental Sciences Faculty Articles
A DNA Tool for the Identification of Heavily Exploited Atlantic Billfishes
Document Type
Article
Publication Date
12-2015
Publication Title
Conservation Genetic Resources
Keywords
COI, PCR-RFLP, Genetics, Fisheries management, Pelagic fish, Roundscale spearfish, Istiophoridae, Perspective, Georgii, Program, Fishes, Marlin
ISSN
1877-7252
Volume
7
Issue/No.
4
First Page
807
Last Page
809
Abstract
Due to the morphological similarities among species, the International Commission for the Conservation of the Atlantic Tunas has recommended the use of diagnostic molecular tools to allow for robust species-level identifications of the billfishes. In this study, a protocol for the molecular identification of all six Atlantic billfishes was developed utilizing a PCR–RFLP approach, targeting the mitochondrial gene cytochrome c oxidase subunit 1. A survey of 28 restriction endonucleases identified two enzymes (TaqI and HaeIII) that produced species-specific banding patterns sufficient to distinguish species. The protocol was validated against billfishes captured across their Atlantic distributions.
NSUWorks Citation
Rodrigo R. Domingues, Gabriel Okuda, Andrea Bernard, Alberto F. Amorim, Juliana De Biasi, and Alexandre W. S. Hilsdorf. 2015. A DNA Tool for the Identification of Heavily Exploited Atlantic Billfishes .Conservation Genetic Resources , (4) : 807 -809. https://nsuworks.nova.edu/occ_facarticles/744.
DOI
10.1007/s12686-015-0489-4
Comments
©Springer Science+Business Media Dordrecht 2015