A DNA Tool for the Identification of Heavily Exploited Atlantic Billfishes

Authors

Rodrigo R. Domingues, Univ Estadual Paulista UNESP - Brazil
Gabriel Okuda, Univ Mogi das Cruzes - Brazil
Andrea Bernard, Nova Southeastern UniversityFollow
Alberto F. Amorim, Instituto de Pesca - Brazil
Juliana De Biasi, Universidade de Mogi das Cruzes - Brazil
Alexandre W. S. Hilsdorf, Universidade de Mogi das Cruzes - Brazil

Document Type

Article

Publication Title

Conservation Genetic Resources

ISSN

1877-7252

Publication Date

12-2015

Keywords

COI, PCR-RFLP, Genetics, Fisheries management, Pelagic fish, Roundscale spearfish, Istiophoridae, Perspective, Georgii, Program, Fishes, Marlin

Abstract

Due to the morphological similarities among species, the International Commission for the Conservation of the Atlantic Tunas has recommended the use of diagnostic molecular tools to allow for robust species-level identifications of the billfishes. In this study, a protocol for the molecular identification of all six Atlantic billfishes was developed utilizing a PCR–RFLP approach, targeting the mitochondrial gene cytochrome c oxidase subunit 1. A survey of 28 restriction endonucleases identified two enzymes (TaqI and HaeIII) that produced species-specific banding patterns sufficient to distinguish species. The protocol was validated against billfishes captured across their Atlantic distributions.

DOI

10.1007/s12686-015-0489-4

Volume

7

Issue

4

First Page

807

Last Page

809

Comments

©Springer Science+Business Media Dordrecht 2015

NSUWorks Citation

Rodrigo R. Domingues, Gabriel Okuda, Andrea Bernard, Alberto F. Amorim, Juliana De Biasi, and Alexandre W. S. Hilsdorf. 2015. A DNA Tool for the Identification of Heavily Exploited Atlantic Billfishes .Conservation Genetic Resources , (4) : 807 -809. https://nsuworks.nova.edu/occ_facarticles/744.