Development and Characterization of 11 Novel Microsatellite Loci for the Roundscale Spearfish Tetrapturus georgii and Their Cross-Species Amplification Among Other Istiophorid Species

Authors

Andrea M. Bernard, Nova Southeastern UniversityFollow
Kevin A. Feldheim, Pritzker Laboratory for Molecular Systematics and Evolution
Mahmood S. Shivji, Nova Southeastern UniversityFollow

ResearcherID

G-4080-2013

Document Type

Article

Publication Title

Journal of Fish Biology

ISSN

0022-1112

Publication Date

10-1-2012

Keywords

Atlantic, Billfish, Istiophoridae, Population genetics, Primer sequence

Abstract

Eleven novel polymorphic microsatellite loci were developed and characterized for the recently validated roundscale spearfish Tetrapturus georgii. Characterization of these markers, based on 35 roundscale spearfish from the western North Atlantic, revealed two to 21 alleles per locus with an average expected heterozygosity (HE) of 0·09–0·94, and all loci conformed to Hardy–Weinberg expectations. Cross-amplification of these 11 loci against all other eight known istiophorid species indicates promising prospects for the utility of these markers for istiophorids in general.

DOI

10.1111/j.1095-8649.2012.03442.x

Volume

81

Issue

5

First Page

1781

Last Page

1786

Comments

©2012 The Authors

NSUWorks Citation

Andrea M. Bernard, Kevin A. Feldheim, and Mahmood S. Shivji. 2012. Development and Characterization of 11 Novel Microsatellite Loci for the Roundscale Spearfish Tetrapturus georgii and Their Cross-Species Amplification Among Other Istiophorid Species .Journal of Fish Biology , (5) : 1781 -1786. https://nsuworks.nova.edu/occ_facarticles/359.