Marine & Environmental Sciences Faculty Articles
Development and Characterization of Fifteen Novel Microsatellite Loci for the Nassau Grouper (Epinephelus striatus) and Their Utility for Cross-Amplification on a Suite of Closely Related Species
Document Type
Article
Publication Date
12-1-2012
Publication Title
Conservation Genetics Resources
Keywords
Grouper genetics, Grouper conservation, Serranidae, Epinephelus, Mycteroperca
ISSN
1877-7252
Volume
4
Issue/No.
4
First Page
983
Last Page
986
Abstract
The Nassau grouper (Epinephelus striatus), a severely overexploited and endangered coral reef fish in the Western Atlantic, is in urgent need of conservation and management measures. We report the development and characterization of 15 polymorphic, mostly tetranucleotide microsatellite loci based on 40 Nassau grouper samples collected from two Caribbean spawning aggregations. The number of alleles per locus and expected heterozygosities ranged from 4 to 19 and 0.40 to 0.92, respectively. We also demonstrate the cross-amplification utility of these microsatellites for genetic studies of five other grouper species of conservation interest.
Additional Comments
US Geological Survey Project #: 07ERAG0078; NOAA Center for Sponsored Coastal Ocean Science award #: NA10NOS4260221
NSUWorks Citation
Andrea M. Bernard, Kevin A. Feldheim, Vincent P. Richards, Richard S. Nemeth, and Mahmood S. Shivji. 2012. Development and Characterization of Fifteen Novel Microsatellite Loci for the Nassau Grouper (Epinephelus striatus) and Their Utility for Cross-Amplification on a Suite of Closely Related Species .Conservation Genetics Resources , (4) : 983 -986. https://nsuworks.nova.edu/occ_facarticles/358.
ResearcherID
G-4080-2013
DOI
10.1007/s12686-012-9688-4
Comments
©Springer Science+Business Media B.V. 2012