Title

Development and Characterization of Fifteen Novel Microsatellite Loci for the Nassau Grouper (Epinephelus striatus) and Their Utility for Cross-Amplification on a Suite of Closely Related Species

Document Type

Article

Publication Date

12-1-2012

Publication Title

Conservation Genetics Resources

Keywords

Grouper genetics, Grouper conservation, Serranidae, Epinephelus, Mycteroperca

ISSN

1877-7252

Volume

4

Issue/No.

4

First Page

983

Last Page

986

Abstract

The Nassau grouper (Epinephelus striatus), a severely overexploited and endangered coral reef fish in the Western Atlantic, is in urgent need of conservation and management measures. We report the development and characterization of 15 polymorphic, mostly tetranucleotide microsatellite loci based on 40 Nassau grouper samples collected from two Caribbean spawning aggregations. The number of alleles per locus and expected heterozygosities ranged from 4 to 19 and 0.40 to 0.92, respectively. We also demonstrate the cross-amplification utility of these microsatellites for genetic studies of five other grouper species of conservation interest.

Comments

©Springer Science+Business Media B.V. 2012

Additional Comments

US Geological Survey Project #: 07ERAG0078; NOAA Center for Sponsored Coastal Ocean Science award #: NA10NOS4260221

ResearcherID

G-4080-2013

DOI

10.1007/s12686-012-9688-4

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