Marine & Environmental Sciences Faculty Articles
ORCID
https://orcid.org/0000-0001-8385-7066
Document Type
Article
Publication Title
Frontiers in Marine Science
ISSN
2296-7745
Publication Date
9-27-2021
Keywords
betaine lipids, glycolipids, Symbiodiniaceae, tocomonoenol, coral metabolome
Abstract
Stony coral tissue loss disease, first observed in Florida in 2014, has now spread along the entire Florida Reef Tract and on reefs in many Caribbean countries. The disease affects a variety of coral species with differential outcomes, and in many instances results in whole-colony mortality. We employed untargeted metabolomic profiling of Montastraea cavernosa corals affected by stony coral tissue loss disease to identify metabolic markers of disease. Herein, extracts from apparently healthy, diseased, and recovered Montastraea cavernosa collected at a reef site near Ft. Lauderdale, Florida were subjected to liquid-chromatography mass spectrometry-based metabolomics. Unsupervised principal component analysis reveals wide variation in metabolomic profiles of healthy corals of the same species, which differ from diseased corals. Using a combination of supervised and unsupervised data analyses tools, we describe metabolite features that explain variation between the apparently healthy corals, between diseased corals, and between the healthy and the diseased corals. By employing a culture-based approach, we assign sources of a subset of these molecules to the endosymbiotic dinoflagellates, Symbiodiniaceae. Specifically, we identify various endosymbiont- specific lipid classes, such as betaine lipids, glycolipids, and tocopherols, which differentiate samples taken from apparently healthy corals and diseased corals. Given the variation observed in metabolite fingerprints of corals, our data suggests that metabolomics is a viable approach to link metabolite profiles of different coral species with their susceptibility and resilience to numerous coral diseases spreading through reefs worldwide.
DOI
doi: 10.3389/fmars.2021.714778
Volume
8
First Page
714778
Additional Comments
This article is part of the Research Topic Stony Coral Tissue Loss Disease in the Caribbean View all 39 Articles: https://www.frontiersin.org/research-topics/16501/stony-coral-tissue-loss-disease-in-the-caribbean#articles
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
NSUWorks Citation
Jessica M. Deutsch, Olakunle A. Jaiyesimi, Kelly A. Pitts, Jay Houk, Blake Ushijima, Brian K. Walker, Valerie J. Paul, and Neha Garg. 2021. Metabolomics of Healthy and Stony Coral Tissue Loss Disease Affected Montastraea cavernosa Corals .Frontiers in Marine Science : 714778 . https://nsuworks.nova.edu/occ_facarticles/1337.
Comments
Copyright © 2021 Deutsch, Jaiyesimi, Pitts, Houk, Ushijima, Walker, Paul and Garg. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms