Characterization of fungal biodiversity and communities associated with reef macroalga Sargassum ilicifolium reveals fungal community differentiation according to geographic locality and algal structure

Authors

Benjamin J. Wainwright, National University of Singapore
Andrew G. Bauman, National University of SingaporeFollow
Geoffrey L. Zahn, Utah Valley University
Peter A. Todd, National University of Singapore
Danwei Huang, National University of Singapore

ORCID

0000-0001-9260-2153

Document Type

Article

Publication Title

Marine Biodiversity

ISSN

1867-1624

Publication Date

8-5-2019

Keywords

Coral reefs, Fungal ITS, Marine fungi, Microbiome, Singapore, Southeast Asia

Abstract

Marine environments abound with opportunities to discover new species of fungi even in relatively well-studied ecosystems such as coral reefs. Here, we investigated the fungal communities associated with the canopy forming macroalga Sargassum ilicifolium(Turner) C. Argardh (1820) in Singapore. We collected eight S. ilicifolium thalli from each of eight island locations and separated them into three structures—leaves, holdfast and vesicles. Amplicon sequencing of the fungal internal transcribed spacer 1 (ITS1) and subsequent analyses revealed weak but significant differences in fungal community composition from different structures. Fungal communities were also significantly different among sampling localities, even over relatively small spatial scales (≤ 12 km). Unsurprisingly, all structures from all localities were dominated by unclassified fungi. Our findings demonstrate the potential of marine environments to act as reservoirs of undocumented biodiversity that harbour many novel fungal taxa. These unclassified fungi highlight the need to look beyond terrestrial ecosystems in well-studied regions of the world, and to fully characterize fungal biodiversity in hotspots such as Southeast Asia for better understanding the roles they play in promoting and maintaining life on our planet.

DOI

10.1007/s12526-019-00992-6

Volume

49

First Page

2601

Last Page

2608

Comments

Acknowledgments

We thank Jack Darcy for the R script that allows sequence quality information to be ‘reattached’ to the processed ITSx data. Full script can be found at https://github.com/gzahn/Sargassum_Fungi/blob/master/R/itsx_fastq_extractor.r

Funding

This study was funded by the National Research Foundation, Prime Minister’s Office, Singapore, under its Marine Science R&D Programme (MSRDP-P03) and The Wildlife Reserves Singapore Conservation Fund (WRSCF). The funders had no role in study design, data collection and analysis, decision to publish or preparation of the manuscript.

Data availability

All sequences associated with this work have been deposited at the National Center for Biotechnology Information (https://www.ncbi.nlm.nih.gov/bioproject/) under BioProject ID: PRJNA504438.

Electronic supplementary material

(XLSX 793 kb)

NSUWorks Citation

Benjamin J. Wainwright, Andrew G. Bauman, Geoffrey L. Zahn, Peter A. Todd, and Danwei Huang. 2019. Characterization of fungal biodiversity and communities associated with reef macroalga Sargassum ilicifolium reveals fungal community differentiation according to geographic locality and algal structure .Marine Biodiversity : 2601 -2608. https://nsuworks.nova.edu/occ_facarticles/1281.