Marine & Environmental Sciences Faculty Articles
Seq’ and ARMS shall find: DNA (meta)barcoding of Autonomous Reef Monitoring Structures across the tree of life uncovers hidden cryptobiome of tropical urban coral reefs
ORCID
0000-0001-9260-2153
Document Type
Article
Publication Title
Molecular Ecology
ISSN
0962-1083
Publication Date
6-18-2022
Keywords
cryptobenthic diversity, eukaryotes, high-throughput sequencing, marine biomonitoring, metazoans, microbial
Abstract
Coral reefs are among the richest marine ecosystems on Earth, but there remains much diversity hidden within cavities of complex reef structures awaiting discovery. While the abundance of corals and other macroinvertebrates are known to influence the diversity of other reef-associated organisms, much remains unknown on the drivers of cryptobenthic diversity. A combination of standardized sampling with 12 units of the Autonomous Reef Monitoring Structure (ARMS) and high-throughput sequencing was utilized to uncover reef cryptobiome diversity across the equatorial reefs in Singapore. DNA barcoding and metabarcoding of mitochondrial cytochrome c oxidase subunit I, nuclear 18S and bacterial 16S rRNA genes revealed the taxonomic composition of the reef cryptobiome, comprising 15,356 microbial ASVs from over 50 bacterial phyla, and 971 MOTUs across 15 metazoan and 19 non-metazoan eukaryote phyla. Environmental factors across different sites were tested for relationships with ARMS diversity. Differences among reefs in diversity patterns of metazoans and other eukaryotes, but not microbial communities, were associated with biotic (coral cover) and abiotic (distance, temperature and sediment) environmental variables. In particular, ARMS deployed at reefs with higher coral cover had greater metazoan diversity and encrusting plate cover, with larger-sized non-coral invertebrates influencing spatial patterns among sites. Our study showed that DNA barcoding and metabarcoding of ARMS constitute a valuable tool for quantifying cryptobenthic diversity patterns and can provide critical information for the effective management of coral reef ecosystems.
DOI
10.1111/mec.16568
NSUWorks Citation
Yin Cheong Aden Ip, Jia Jin Marc Chang, Ren Min Oh, Zheng Bin Randolph Quek, Yong Kit Samuel Chan, Andrew G. Bauman, and Danwei Huang. 2022. Seq’ and ARMS shall find: DNA (meta)barcoding of Autonomous Reef Monitoring Structures across the tree of life uncovers hidden cryptobiome of tropical urban coral reefs .Molecular Ecology . https://nsuworks.nova.edu/occ_facarticles/1267.
Comments
We are extremely grateful to the following for their help in fieldwork and processing: Sudhanshi Sanjeev Jain, Jovena Chun Ling Seah, Zack Chen, Chin Soon Lionel Ng, Joy Shu Yee Wong, Sherlyn Sher Qing Lim, Zhi Ting Yip, and Jun Wei Phua. We also acknowledge the National Supercomputing Centre, Singapore (https://www.nscc.sg) for providing the computational resources for analysis. This research was supported by the Singapore Ministry of Education Academic Research Fund Tier 1 (R-154-000-A63-114), the National Research Foundation, Prime Minister's Office, Singapore under its Marine Science R&D Programme (MSRDP-P03), and an AXA Postdoctoral Fellowship (R-154-000-649-507) to A.G.B.