Marine & Environmental Sciences Faculty Articles

ORCID

0000-0001-8225-8344

Document Type

Article

Publication Title

Journal of Parasitology

ISSN

0022-3395

Publication Date

2-25-2020

Keywords

Experimental infection, Neurophysiology, Fundulus parvipinnis, Euhaplorchis californiensis, Brain stem, Reproductive physiology, Parasite quantification

Abstract

Some parasite species alter the behavior of intermediate hosts to promote transmission to the next host in the parasite's life cycle. This is the case for Euhaplorchis californiensis, a brain-encysting trematode parasite that causes behavioral changes in the California killifish (Fundulus parvipinnis). These manipulations increase predation by the parasite's final host, piscivorous marsh birds. The mechanisms by which E. californiensis achieves this manipulation remain poorly understood. As E. californiensis cysts reside on the surface of the killifish's brain, discerning regional differences in parasite distribution could indicate mechanisms for host control. In this study, we developed a method for repeated experimental infections. In addition, we measured brain-region specific density using a novel methodology to locate and quantify parasite infection. We show that E. californiensis cysts are non-randomly distributed on the fish brain, aggregating on the diencephalon/ mesencephalon region (a brain area involved in controlling reproduction and stress coping) and the rhombencephalon (an area involved in controlling locomotion and basal physiology). Determining causal mechanisms behind this pattern of localization will guide future research examining the neurological mechanisms of parasite-induced host manipulation. These findings suggest that parasites are likely targeting the reproductive, monoaminergic, and locomotor systems to achieve host behavioral manipulation.

DOI

10.1645/19-86

Volume

106

Issue

1

First Page

188

Last Page

197

Comments

©American Society of Parasitologists 2020. This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Additional Comments

Research Council of Norway project #s: 240116, 250048

Peer Reviewed

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