Presentation Title

Preparation and Size Characterization of Niosomal Nanocarriers for Targeted Drug Delivery

Presenter Credentials

Nazanin Kianinejad, College of Pharmacy, Graduate Program in Drug Development Track, third year, Ph.D

Presenter Degree

MS

Co-Author Credentials

Young M. Kwon, Ph.D, Associate Professor

College

College of Pharmacy

Campus Location

Ft. Lauderdale

Format

Poster

IRB Approval Verification

N/A

Abstract

Objective: The goal of this study is to prepare and evaluate niosomal preparations for potential application in drug delivery to the brain. Background: Niosomal nanocarriers are electrically neutral vesicle systems with bilayer membranes and interior cavities. Niosome is favored in the design of drug delivery systems due to their loading capacity, wide surface area, and ability to incorporate hydrophilic and lipophilic components. Niosome is one such system with a hydrated mixture of cholesterol and non-ionic surfactants. Niosomes are pharmaceutically superior to liposomes or their related vesicle systems, due to their higher chemical stability, ease of storage, handling, and cost-effectiveness. Desired size of niosomes for targeted drug delivery is 150 – 300 nm. Methods: Niosomes were prepared based on the thin film hydration method with modifications. Briefly, required amount of nonionic surfactants (Span 60), cholesterol, and chloroform were mixed. Chloroform was then evaporated via a rotoevaporator at 60-65°C under a reduced pressure of 437 mbar to obtain a thin film. Deionized water at 60-65°C was added and sonicated for varying durations to produce niosomes. The size distributions of the aqueous niosomal suspensions were characterized on a zeta-sizer. Results: The mean diameters and the widths of distributions upon 5, 10, and 15 minute-sonication were: 153+/-88 nm (large aggregates present), 137+/-60 nm (large aggregates present), and 185+/-48 nm, respectively. Conclusion: Niosomal nanocarriers with a desired mean diameter and uniform size distribution was successfully prepared for further application in drug loading and targeted delivery for diseased tissues such as brain tumors. Grants: NSU HPD Grant

Selection Criteria

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Preparation and Size Characterization of Niosomal Nanocarriers for Targeted Drug Delivery

Objective: The goal of this study is to prepare and evaluate niosomal preparations for potential application in drug delivery to the brain. Background: Niosomal nanocarriers are electrically neutral vesicle systems with bilayer membranes and interior cavities. Niosome is favored in the design of drug delivery systems due to their loading capacity, wide surface area, and ability to incorporate hydrophilic and lipophilic components. Niosome is one such system with a hydrated mixture of cholesterol and non-ionic surfactants. Niosomes are pharmaceutically superior to liposomes or their related vesicle systems, due to their higher chemical stability, ease of storage, handling, and cost-effectiveness. Desired size of niosomes for targeted drug delivery is 150 – 300 nm. Methods: Niosomes were prepared based on the thin film hydration method with modifications. Briefly, required amount of nonionic surfactants (Span 60), cholesterol, and chloroform were mixed. Chloroform was then evaporated via a rotoevaporator at 60-65°C under a reduced pressure of 437 mbar to obtain a thin film. Deionized water at 60-65°C was added and sonicated for varying durations to produce niosomes. The size distributions of the aqueous niosomal suspensions were characterized on a zeta-sizer. Results: The mean diameters and the widths of distributions upon 5, 10, and 15 minute-sonication were: 153+/-88 nm (large aggregates present), 137+/-60 nm (large aggregates present), and 185+/-48 nm, respectively. Conclusion: Niosomal nanocarriers with a desired mean diameter and uniform size distribution was successfully prepared for further application in drug loading and targeted delivery for diseased tissues such as brain tumors. Grants: NSU HPD Grant