Presentation Title
Evaluation of Gelling Parameters of Alginates on Cell Viability of Umbilical Cord Stem Cells
Format
Poster
Start Date
10-2-2012 12:00 AM
Abstract
Objective. To assess ionogelling, ion concentration, and composition of pharmaceutical grade alginates on viability of human umbilical cord derived mesenchymal stem cells (hUMSCs). Background. Alginate is a widely used biomaterial due to its ionogelling property, therefore evaluation of gelling parameters of alginate is important for its use in tissue engineering. Methods. Two pharmaceutical grade sodium alginates, high and low guluronate (HG and LG) were used in three sets of experiments: 1) cells grown in 12-well plate were exposed to beads prepared by extruding 2% w/v alginate solutions into individual calcium chloride (CaCl2) solutions (50, 100, 150 and 200 mM); 2) the hUMSCs grown in similar culture conditions were exposed to 30 mg of CaCl2 (10, 50, 100 and 200 mM) solutions; and 3) cells exposed to non-crosslinked HG or LG alginates. The cell viability was estimated by MTT assay. A student’s test was used to evaluate the data and P < 0.05 was considered statistically significant. Results. The cell viability in 39 the presence of alginate beads crosslinked with 50 and 100mM CaCl2 solutions was comparable to control, and decreased significantly at higher concentrations. The cells treated with CaCl2 solutions were comparable to control at all concentrations. The cell viability was significantly high in HG than LG alginate solution. Conclusion. High hUMSCs survival rate in the presence of alginate gels was primarily dependent on the alginate backbone structure (G content), and the concentration of calcium during ionogelation. The results suggest feasibility of using G rich alginates for tissue engineering purposes. Grant. This study was funded by the Chancellor’s Faculty Research and Development Grant.
Evaluation of Gelling Parameters of Alginates on Cell Viability of Umbilical Cord Stem Cells
Objective. To assess ionogelling, ion concentration, and composition of pharmaceutical grade alginates on viability of human umbilical cord derived mesenchymal stem cells (hUMSCs). Background. Alginate is a widely used biomaterial due to its ionogelling property, therefore evaluation of gelling parameters of alginate is important for its use in tissue engineering. Methods. Two pharmaceutical grade sodium alginates, high and low guluronate (HG and LG) were used in three sets of experiments: 1) cells grown in 12-well plate were exposed to beads prepared by extruding 2% w/v alginate solutions into individual calcium chloride (CaCl2) solutions (50, 100, 150 and 200 mM); 2) the hUMSCs grown in similar culture conditions were exposed to 30 mg of CaCl2 (10, 50, 100 and 200 mM) solutions; and 3) cells exposed to non-crosslinked HG or LG alginates. The cell viability was estimated by MTT assay. A student’s test was used to evaluate the data and P < 0.05 was considered statistically significant. Results. The cell viability in 39 the presence of alginate beads crosslinked with 50 and 100mM CaCl2 solutions was comparable to control, and decreased significantly at higher concentrations. The cells treated with CaCl2 solutions were comparable to control at all concentrations. The cell viability was significantly high in HG than LG alginate solution. Conclusion. High hUMSCs survival rate in the presence of alginate gels was primarily dependent on the alginate backbone structure (G content), and the concentration of calcium during ionogelation. The results suggest feasibility of using G rich alginates for tissue engineering purposes. Grant. This study was funded by the Chancellor’s Faculty Research and Development Grant.