Department of Physical Therapy Faculty Articles
Title
Simultaneous Delivery of Chemotherapeutic and Thermal-Optical Agents to Cancer Cells by a Polymeric (PLGA) Nanocarrier: An in Vitro Study.
ISBN or ISSN
0724-8741
Volume
27
Issue
10
Publication Date / Copyright Date
10-1-2010
First Page
2242
Last Page
2253
DOI Number
10.1007/s11095-010-0231-6
Abstract
PURPOSE:
To test the effectiveness of a dual-agent-loaded PLGA nanoparticulate drug delivery system containing doxorubicin (DOX) and indocyanine green (ICG) in a DOX-sensitive cell line and two resistant cell lines that have different resistance mechanisms.
METHODS:
The DOX-sensitive MES-SA uterine sarcoma cell line was used as a negative control. The two resistant cell lines were uterine sarcoma MES-SA/Dx5, which overexpresses the multidrug resistance exporter P-glycoprotein, and ovarian carcinoma SKOV-3, which is less sensitive to doxorubicin due to a p53 gene mutation. The cellular uptake, subcellular localization and cytotoxicity of the two agents when delivered via nanoparticles (NPs) were compared to their free-form administration.
RESULTS:
The cellular uptake and cytotoxicity of DOX delivered by NPs were comparable to the free form in MES-SA and SKOV-3, but much higher in MES-SA/Dx5, indicating the capability of the NPs to overcome P-glycoprotein resistance mechanisms. NP-encapsulated ICG showed slightly different subcellular localization, but similar fluorescence intensity when compared to free ICG, and retained the ability to generate heat for hyperthermia delivery.
CONCLUSION:
The dual-agent-loaded system allowed for the simultaneous delivery of hyperthermia and chemotherapy, and this combinational treatment greatly improved cytotoxicity in MES-SA/Dx5 cells and to a lesser extent in SKOV-3 cells.
Disciplines
Medicine and Health Sciences
Keywords
Antineoplastic Agents, Biological Transport, Cell Line, Cell Proliferation, Doxorubicin, Neoplasm, Female, Humans, Lactic Acid, Electron, Nanoparticles, Optical Phenomena, Organic Chemicals, Polyglycolic Acid
NSUWorks Citation
Tang, Yuan; Lei, Tingjun; Manchanda, Romila; Nagesetti, Abhignyan; Fernandez-Fernandez, Alicia; Srinivasan, Supriya; and McGoron, Anthony J., "Simultaneous Delivery of Chemotherapeutic and Thermal-Optical Agents to Cancer Cells by a Polymeric (PLGA) Nanocarrier: An in Vitro Study." (2010). Department of Physical Therapy Faculty Articles. 100.
https://nsuworks.nova.edu/hpd_pt_facarticles/100
