Department of Occupational Therapy Program Faculty Articles
Downregulation of Protein Kinase C and Insulin Stimulated 2-Deoxyglucose uptake in Rat Adipocytes by Phorbol Esters, Glucose and Insulin.
Publication Title
Diabetes
ISSN
0012-1797
Abstract
Phorbol esters translocatively activate and subsequently downregulate protein kinase C and insulin-stimulated glucose uptake in rat adipocytes. This study examined the possibility that other translocative activators of protein kinase C in rat adipocytes, e.g., insulin and glucose, provoke similar downregulating effects. Pretreatment of rat adipocytes for 20-24 h with phorbol esters, 3 nM insulin, 20 mM glucose, or 3 nM insulin plus 20 mM glucose resulted in concomitant decreases in protein kinase C and insulin-stimulated (or phorbol ester-stimulated) [3H]-2-deoxyglucose uptake. Downregulating effects of glucose on protein kinase C and insulin-stimulated [3H]-2-deoxyglucose uptake were also evident within 30 min in adipocytes freshly incubated in medium containing 5-20 mM, rather than 0, glucose. These findings confirm that protein kinase C is required during insulin-stimulated glucose uptake and raise the possibility that downregulation of protein kinase C by continued translocative activation of the enzyme may contribute (along with other factors) to impaired responsiveness of the glucose transport system after prolonged insulin and/or glucose treatment.
Volume
40
First Page
1274
Last Page
1281
Disciplines
Medicine and Health Sciences
NSUWorks Citation
Ishizuka, T.; Cooper, D. R.; Arnold, Thomas Paul; Hermandez, H.; and Farese, R. V., "Downregulation of Protein Kinase C and Insulin Stimulated 2-Deoxyglucose uptake in Rat Adipocytes by Phorbol Esters, Glucose and Insulin." (2017). Department of Occupational Therapy Program Faculty Articles. 60.
https://nsuworks.nova.edu/hpd_ot_faculty_articles/60
