NSU-MD Faculty Articles
Measurements of coronary plasma and pericardial infusate adenosine concentrations during exercise in conscious dog: relationship to myocardial oxygen consumption and coronary blood flow.
Publication Title
Journal of molecular and cellular cardiology
Publisher
Academic Press
ISSN
0022-2828
Publication Date
10-1-1983
Keywords
Adenosine, Animals, Coronary Circulation, Dogs, Female, Male, Myocardium, Oxygen Consumption, Pericardium, Physical Exertion
Abstract
A conscious dog model was employed to evaluate the relationship among myocardial oxygen consumption (MVO2), myocardial adenosine release and coronary blood flow (CNF) during graded treadmill exercise. Two methods were utilized simultaneously as indexes of changes in interstitial adenosine concentrations, (1) a pericardial infusate technique and (2) to measurement of arterial-coronary sinus plasma adenosine concentration differences. Graded exercise was associated with graded increases in MVO2, CBF, pericardial infusate adenosine concentration (PI Ado) and adenosine washout in coronary plasma. Regression analysis demonstrated significant linear relationships for MVO2 v. CBF (r = 0.78, P less than 0.01), MVO2 v. PI Ado (r = 0.52, P less than 0.01), and PI Ado v. CBF (r = 0.76, P less than 0.01). Coronary plasma adenosine arterio-sinus differences, sinus plasma concentrations, and net washout of adenosine also increased with graded exercise, however, a significant inter-animal variance was noted. These data suggest that the plasma adenosine assay is capable of detecting directional (qualitative) changes associated with changes in cardiac metabolism, however, it may not be reliable as a quantitative indicator of interstitial adenosine concentrations due to multiple factors which may influence the plasma adenosine pool. The pericardial infusate technique, which presumably represents a model of diffusion, is relatively invariant by comparison. The results demonstrate a significant correlation among MVO2, PI Ado and CBF, and thereby provide support to the hypothesis that adenosine is a major factor in the coupling of myocardial oxygen demand to oxygen supply under physiological conditions.
DOI
10.1016/0022-2828(83)90257-2
Volume
15
Issue
10
First Page
673
Last Page
683
Disciplines
Medicine and Health Sciences
NSUWorks Citation
Ely, Stephen W.; Knabb, R M; Bacchus, A N; Rubio, R; and Berne, R M, "Measurements of coronary plasma and pericardial infusate adenosine concentrations during exercise in conscious dog: relationship to myocardial oxygen consumption and coronary blood flow." (1983). NSU-MD Faculty Articles. 88.
https://nsuworks.nova.edu/hpd_md_facarticles/88
