NSU-MD Faculty Articles

Title

Expression of pluripotent stem cell reprogramming factors by prostate tumor initiating cells.

ISBN or ISSN

0022-5347

Publication Title

The Journal of urology

Volume

183

Issue

5

Publication Date / Copyright Date

5-1-2010

First Page

2045

Last Page

2053

Publisher

Elsevier Inc.

DOI Number

10.1016/j.juro.2009.12.092

Abstract

PURPOSE: We identified a discrete population of stem cell-like tumor cells expressing 5 essential transcription factors required to reprogram pluripotency in prostate tumor cell lines and primary prostate cancer tissue.

MATERIALS AND METHODS: DU145 and PC3 human prostate cancer cell lines (ATCC), tumor tissue from patients with prostate cancer and normal prostate tissue were evaluated for the reprogramming factors OCT3/4 (Cell Signaling Technology), SOX2, Klf4 (Santa Cruz Biotechnology, Santa Cruz, California), Nanog (BioLegend) and c-Myc (Cell Signaling) by semiquantitative reverse transcriptase-polymerase chain reaction, histological and immunohistochemical analysis. Stem cell-like tumor cells were enriched by flow cytometric cell sorting using E-cadherin (R&D Systems) as a surface marker, and soft agar, spheroid and tumorigenicity assays to confirm cancer stem cell-like characteristics.

RESULTS: mRNA expression of transcription factors OCT3/4 and SOX2 highly correlated in primary prostate tumor tissue samples. The number of OCT3/4 or SOX2 expressing cells was significantly increased in prostate cancer tissue compared to that in normal prostate or benign prostate hyperplasia tissue (p

CONCLUSIONS: Data suggest that prostate tumor cells expressing pluripotent stem cell transcription factors are highly tumorigenic. Identifying such cells and their importance in prostate cancer growth could provide opportunities for novel targeting strategies for prostate cancer therapy.

Disciplines

Medicine and Health Sciences

Keywords

Adult, Analysis of Variance, Animals, Blotting, Western, Cell Line, Tumor, DNA-Binding Proteins, Flow Cytometry, Gene Expression, Homeodomain Proteins, Humans, Immunohistochemistry, Kruppel-Like Transcription Factors, Male, Mice, Mice, SCID, Middle Aged, Nanog Homeobox Protein, Octamer Transcription Factor-3, Pluripotent Stem Cells, Prostatic Neoplasms, RNA, Messenger, Reverse Transcriptase Polymerase Chain Reaction, SOXB1 Transcription Factors, Transcription Factors, Tumor Cells, Cultured

Peer Reviewed

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