Document Type
Article
Publication Date
1-1-2013
Publication Title
International Journal of Medical Sciences
Keywords
adeno-associated viral vector, adenoviral vector, cardiovascular control regions of brain, gene transfer., neuron
ISSN
1449-1907
Volume
10
Issue/No.
5
First Page
607
Last Page
16
Abstract
Viral vectors have been utilized extensively to introduce genetic material into the central nervous system. In order to investigate gene functions in cardiovascular control regions of rat brain, we applied WPRE (woodchuck hepatitis virus post-transcriptional regulatory element) enhanced-adenoviral (Ad) and adeno-assoicated virus (AAV) type 2 vectors to mediate neuronal gene delivery to the paraventricular nucleus of the hypothalamus, the nucleus tractus solitarius and the rostral ventrolateral medulla, three important cardiovascular control regions known to express renin-angiotensin system (RAS) genes. Ad or AAV2 harboring an enhanced green fluorescent protein (EGFP) reporter gene or the angiotensin type 2 receptor gene were microinjected into these brain regions in adult rats. Our results demonstrated that both AAV2 and Ad vectors elicited long-term neuronal transduction in these regions. Interestingly, we found that the WPRE caused expression of GFP driven by the synapsin1 promoter in pure glial cultures or co-cultures of neurons and glia derived from rat hypothalamus and brainstem. However, in rat paraventricular nucleus WPRE did not cause expression of GFP in glia. This demonstrates the potential use of these vectors in studies of physiological functions of certain genes in the cardiovascular control regions of the brain.
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 License.
NSUWorks Citation
Zhang, Yanling; Gao, Yongxin; Speth, Robert C.; Jiang, Nan; Mao, Yingying; Sumners, Colin; and Li, Hongwei, "Adenoviral and adeno-associated viral vectors-mediated neuronal gene transfer to cardiovascular control regions of the rat brain" (2013). HPD Articles. 91.
https://nsuworks.nova.edu/hpd_facarticles/91
ORCID ID
0000-0002-6434-2167
DOI
10.7150/ijms.5700
Copyright
© Ivyspring International Publisher.
