Document Type
Article
Publication Date
6-13-2023
Publication Title
Microbiology Research
Keywords
titanium oxides, KOH treatment, dendritic cell, Mycobacterium leprae, leprosy
ISSN
2036-7481
Volume
14
First Page
787
Last Page
797
Abstract
The two polar clinical forms of leprosy, termed tuberculoid and lepromatous, have polarized cellular immune responses with complex immunological distinctions. The predominance of DCs in tuberculoid leprosy has been reported, while the lepromatous pattern of illness is associated with weak activation of local populations of DCs. TiO2 nanoparticles have previously been shown to induce maturation of these cells, leading to an inflammatory response similar to adjuvant usage in vaccine administration. We aimed to evaluate the effect of potassium-incorporated Ti oxide nanostructures, namely KTiOxs, in the response of human monocyte-derived DCs to live M. leprae. Human monocytic cell line dual THP-1, which harbors two inducible reporter plasmid systems for transcription factor activation of NF-κB and interferon regulating factor (IRF), was treated with titanium control or with 1 mol/L KOH-treated Ti or 10 mol/L KOH for 24 h. Subsequently, cells were infected with M. leprae. KTiOx nanoparticles increase DC phagocytic activity without inflammation. KTiOx exposure of DCs led to an increase in IRF activation with modulation of the inflammatory response to live M. leprae. It also led to differential secretion of the critical components of innate immune response and the development of cell-mediated immunity against intracellular pathogens. This study demonstrates the effect of nanostructures of KTiOxs and the usefulness of nanoparticle technology in the in vitro activation of human DCs against an infectious disease with a puzzling immune spectrum. Our findings may prompt future therapeutic strategies, such as DC immunotherapy for disseminated and progressive lepromatous lesions
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
NSUWorks Citation
Warren, Sam; Yoon Lee, So; Barragan, Jose; Kositangool, Piya; Yoshikubo, Hatsuko; and Cervantes, Jorge, "Potassium-Incorporated Titanium Oxide Nanoparticles Modulate Human Dendritic Cell Immune Response to Mycobacterium leprae" (2023). HPD Articles. 499.
https://nsuworks.nova.edu/hpd_facarticles/499
DOI
10.3390/microbiolres14020055
Copyright
© 2023 by the authors.
