Preliminary analysis of the buccal mucosal fungal microbiome in oral lichen planus patients.
Document Type
Article
Publication Date
8-1-2023
Publication Title
Journal of Oral Pathology & Medicine
Keywords
mucosal mycobiome, next-generation sequencing, oral lichen planus
ISSN
1600-0714
Volume
52
Issue/No.
7
First Page
680
Last Page
690
Abstract
OBJECTIVE: To determine the structure and co-occurrence patterns of mucosal fungal community in oral lichen planus (OLP) patients.
SUBJECTS AND METHODS: Mucosal swab samples from 20 OLP patients and 10 healthy controls (HCs) were collected and the mucosal mycobiomes were sequenced. The abundance, frequency, and diversity of fungi were analyzed, as well as the inter-genera interactions. The associations between fungal genera and OLP severity were further identified.
RESULTS: At the genus level, the relative abundance of unclassified_Trichocomaceae was significantly decreased in the reticular and erosive OLP groups compared to HCs. Meanwhile, significantly lower levels of Pseudozyma were observed in the reticular OLP group compared to HCs. The negative:positive cohesiveness ratio was significantly lower in the OLP group than HCs, indicating a relatively unstable fungal ecological system in the OLP group. In the OLP group, the abundance of unclassified_Nectriaceae was significantly correlated with the reticulation/erythema/ulceration (REU) score.
CONCLUSIONS: Compared to HCs, the decreased stability of fungal communities and the decreased abundances of two genera (unclassified_Trichocomaceae and Pseudozyma) on buccal mucosa were identified in OLP patients.
NSUWorks Citation
Du, Guanhuan; Jiang, Xinke; Han, Xiaozhe; Pan, Lei; and Tang, Guoyao, "Preliminary analysis of the buccal mucosal fungal microbiome in oral lichen planus patients." (2023). HPD Articles. 470.
https://nsuworks.nova.edu/hpd_facarticles/470
DOI
10.1111/jop.13439
Copyright
© 2023 John Wiley & Sons A/S.
Comments
ACKNOWLEDGMENTS This work was supported by the National Natural Science Foundationof China (82101008 and 82020108010), the Innovative ResearchTeam of High-Level Local Universities in Shanghai (SHSMU-ZLCX20212401), and an Intrahospital Research Grant (XK2019007).
ETHICS STATEMENT This study was approved by the ethics committee of the Ninth Peo-ple's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine (No. SH9H-(2016)123). Written informed consent was obtained from all participants before enrollment.