Protective effects of the resveratrol analog piceid in dopaminergic SH-SY5Y cells

Document Type

Article

Publication Date

2-1-2018

Publication Title

Archives of Toxicology

Keywords

Antioxidant, Apoptosis, Bcl-2, Dopamine, ERK1/2, ERK5, Oxidative stress, Phytochemicals, Piceid, Polydatin, Resveratrol, SH-SY5Y, Toxicity caspase

ISSN

0340-5761

Volume

92

Issue/No.

2

First Page

669

Last Page

677

Abstract

Age-related motor deficits, such as loss of balance and coordination, are caused, in part, by loss of dopaminergic neurons. Oxidative stress is known to play a role in this neuronal loss. Resveratrol, a natural antioxidant with anticancer and anti-inflammatory potential, has been shown to protect dopaminergic-like cells (SH-SY5Y) against oxidative stress. However, the low bioavailability of resveratrol makes it worthwhile to explore newer compounds with similar properties. Piceid (RV8), an analog of resveratrol, has greater bioavailability than resveratrol, and our studies found that piceid (10, 20, 30 µM) protects SH-SY5Y cells against oxidative stress. Our investigations also found that the neuroprotection afforded by piceid was decreased when the MAP kinases, ERK1/2 and ERK5, were independently inhibited. Since oxidative stress is considered a master operator of apoptosis, our study also scrutinized dopamine-induced apoptosis and whether caspase-3/7 and Bcl-2 are involved, following piceid pretreatment followed by dopamine exposure. Our findings suggested that piceid pretreatment inhibited the dopamine-induced increase in caspase-3/7 activity and dopamine-induced loss of Bcl-2 expression. Overall, these findings suggest that the neuroprotective effects of piceid are mediated via the activation of ERK1/2, ERK5, and inhibition of apoptosis caused by oxidative stress.

ORCID ID

0000-0003-4970-9857

DOI

10.1007/s00204-017-2073-z

Link to Full Text
Peer Reviewed

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