Alterations in GHRH binding and GHRH receptor mRNA in the pituitary of adult dw/dw rats
Document Type
Article
Publication Date
8-1-2002
Publication Title
Peptides
ISSN
0196-9781
Volume
23
Issue/No.
8
First Page
1497
Last Page
502
Abstract
Lewis dwarf (dw/dw) rats exhibit growth hormone (GH) deficiency and growth retardation linked to a malfunction of GHRH signaling. In this study, GHRH-receptor (GHRH-R) binding and mRNA in the pituitary of adult male dw/dw and age-matched normal Lewis rats was measured by radioligand binding assay and real-time PCR. Only one of nine pools of dw/dw pituitary membranes revealed detectable binding of [His(1), 125I-Tyr(10), Nle(27)]hGHRH(1-32) amide (B(max); 4.3 fmol/mg protein). In contrast, GHRH-R binding was 22.4 +/- 2.60 fmol/mg protein in normal Lewis rats. mRNA for GHRH-R was detectable in all dw/dw rat pituitaries examined, averaging 21% that of Lewis rats. Low expression of GHRH-R reflects reduced GHRH-R mRNA as well as a possible reduction in translation of the receptor protein.
NSUWorks Citation
Gardi, János; Speth, Robert C.; Taishi, Ping; Kacsóh, Bálint; Obál, Ferenc; and Krueger, James M., "Alterations in GHRH binding and GHRH receptor mRNA in the pituitary of adult dw/dw rats" (2002). HPD Articles. 107.
https://nsuworks.nova.edu/hpd_facarticles/107
ORCID ID
0000-0002-6434-2215
DOI
10.1016/s0196-9781(02)00088-8
Copyright
Copyright 2002 Elsevier Science Inc.
