Epigenetic effects of casein-derived opioid peptides in SH-SY5Y human neuroblastoma cells
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Nutrition & Metabolism
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Casein-free, gluten-free diets have been reported to mitigate some of the inflammatory gastrointestinal and behavioral traits associated with autism, but the mechanism for this palliative effect has not been elucidated. We recently showed that the opioid peptide beta-casomorphin-7, derived from bovine (bBCM7) milk, decreases cysteine uptake, lowers levels of the antioxidant glutathione (GSH) and decreases the methyl donor S-adenosylmethionine (SAM) in both Caco-2 human GI epithelial cells and SH-SY5Y human neuroblastoma cells. While human breast milk can also release a similar peptide (hBCM-7), the bBCM7 and hBCM-7 vary greatly in potency; as the bBCM-7 is highly potent and similar to morphine in it's effects. Since SAM is required for DNA methylation, we wanted to further investigate the epigenetic effects of these food-derived opioid peptides. In the current study the main objective was to characterize functional pathways and key genes responding to DNA methylation effects of food-derived opioid peptides.
Medicine and Health Sciences | Pharmacy and Pharmaceutical Sciences
Epigenetics, Gluten free casein free diet, Autism, Glutathione, Gastrointestinal, Inflammation
Trivedi, Malav Suchin; Hodgson, Nathaniel; Walker, Stephen J.; Trooskens, Geert; Nair, Vineeth; and Deth, Richard Carlton, "Epigenetic effects of casein-derived opioid peptides in SH-SY5Y human neuroblastoma cells" (2015). Faculty Articles. 287.