Faculty Articles

Title

Epigenetic effects of casein-derived opioid peptides in SH-SY5Y human neuroblastoma cells

ISBN or ISSN

1743-7075

Publication Title

Nutrition & Metabolism

Volume

12

Issue

54

Publication Date / Copyright Date

12-2015

First Page

1

Last Page

10

Publisher

Springer Nature

DOI Number

10.1186/s12986-015-0050-1

Abstract

Casein-free, gluten-free diets have been reported to mitigate some of the inflammatory gastrointestinal and behavioral traits associated with autism, but the mechanism for this palliative effect has not been elucidated. We recently showed that the opioid peptide beta-casomorphin-7, derived from bovine (bBCM7) milk, decreases cysteine uptake, lowers levels of the antioxidant glutathione (GSH) and decreases the methyl donor S-adenosylmethionine (SAM) in both Caco-2 human GI epithelial cells and SH-SY5Y human neuroblastoma cells. While human breast milk can also release a similar peptide (hBCM-7), the bBCM7 and hBCM-7 vary greatly in potency; as the bBCM-7 is highly potent and similar to morphine in it's effects. Since SAM is required for DNA methylation, we wanted to further investigate the epigenetic effects of these food-derived opioid peptides. In the current study the main objective was to characterize functional pathways and key genes responding to DNA methylation effects of food-derived opioid peptides.

Disciplines

Medicine and Health Sciences | Pharmacy and Pharmaceutical Sciences

Keywords

Epigenetics, Gluten free casein free diet, Autism, Glutathione, Gastrointestinal, Inflammation

Peer Reviewed

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