Faculty Articles
Epigenetic effects of casein-derived opioid peptides in SH-SY5Y human neuroblastoma cells
ISBN or ISSN
1743-7075
Publication Title
Nutrition & Metabolism
Volume
12
Issue
54
Publication Date / Copyright Date
12-2015
First Page
1
Last Page
10
Publisher
Springer Nature
DOI Number
10.1186/s12986-015-0050-1
Abstract
Casein-free, gluten-free diets have been reported to mitigate some of the inflammatory gastrointestinal and behavioral traits associated with autism, but the mechanism for this palliative effect has not been elucidated. We recently showed that the opioid peptide beta-casomorphin-7, derived from bovine (bBCM7) milk, decreases cysteine uptake, lowers levels of the antioxidant glutathione (GSH) and decreases the methyl donor S-adenosylmethionine (SAM) in both Caco-2 human GI epithelial cells and SH-SY5Y human neuroblastoma cells. While human breast milk can also release a similar peptide (hBCM-7), the bBCM7 and hBCM-7 vary greatly in potency; as the bBCM-7 is highly potent and similar to morphine in it's effects. Since SAM is required for DNA methylation, we wanted to further investigate the epigenetic effects of these food-derived opioid peptides. In the current study the main objective was to characterize functional pathways and key genes responding to DNA methylation effects of food-derived opioid peptides.
Disciplines
Medicine and Health Sciences | Pharmacy and Pharmaceutical Sciences
Keywords
Epigenetics, Gluten free casein free diet, Autism, Glutathione, Gastrointestinal, Inflammation
NSUWorks Citation
Trivedi, Malav Suchin; Hodgson, Nathaniel; Walker, Stephen J.; Trooskens, Geert; Nair, Vineeth; and Deth, Richard Carlton, "Epigenetic effects of casein-derived opioid peptides in SH-SY5Y human neuroblastoma cells" (2015). Faculty Articles. 287.
https://nsuworks.nova.edu/hpd_corx_facarticles/287
