Faculty Articles
Structural engineering to control density, conformation, and bioactivity of the poly(ethylene glycol)-grafted poly(urethane urea) scaffolds
Publication Title
Journal of Bioactive and Compatible Polymers
Publisher
Sage Publications
ISSN
1530-8030
Publication Date
3-1-2019
Keywords
Gelatin, mesenchymal stem cells, poly(urethane urea), poly(ethylene glycol), scaffold, salt leaching, tissue engineering
Abstract
Poly(urethane urea) scaffolds were fabricated through combined salt leaching and solvent casting methods. The scaffolds were then functionalized via aminolysis with poly(ethylene glycol) (PEG-g-PUU). To compare its bioactivity, gelatin was also grafted onto the aminolyzed poly(urethane urea) surface (Gel-g-PUU). Chemical changes at the surface were then monitored using quantitative/qualitative methods. Grafting with both gelatin and poly(ethylene glycol) remarkably enhanced the wettability of poly(urethane urea). Proliferation of human adipose–derived mesenchymal stem cells on poly(urethane urea) and the modified poly(urethane urea)s was evaluated by 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide assay. The cell experiment results showed that both the modified poly(urethane urea)s enhanced the attachment and proliferation of human adipose–derived mesenchymal stem cells compared to pure poly(urethane urea). Based on previous reports, while a supportive role is observed at adequate poly(ethylene glycol) graft densities, cell adhesion and proliferation are inhibited at very high grafting densities. To correlate the cell data to poly(ethylene glycol) conformations, the surface tension was measured. Data on human adipose–derived mesenchymal stem cells’ attachment/proliferation and contact angle/surface free energy together showed that the grafting density of poly(ethylene glycol) was regulated by optimizing aminolysis conditions, careful selection of poly(ethylene glycol)’s molecular weight, and bulk properties of the matrix poly(urethane urea). As a result, surface overcrowding and brush conformation of the poly(ethylene glycol) chains were avoided, and human adipose–derived mesenchymal stem cell attachment and proliferation occurred on the PEG-g-PUU scaffold at a comparable level to the Gel-g-PUU.
DOI
10.1177/0883911518819224
Volume
34
Issue
2
First Page
209
Last Page
223
Disciplines
Medicine and Health Sciences | Pharmacy and Pharmaceutical Sciences
NSUWorks Citation
Shaneh, Shideh; Shokrolahi, Fatemeh; Shokrollahi, Parvin; Yeganeh, Hamid; and Omidian, Hamid, "Structural engineering to control density, conformation, and bioactivity of the poly(ethylene glycol)-grafted poly(urethane urea) scaffolds" (2019). Faculty Articles. 260.
https://nsuworks.nova.edu/hpd_corx_facarticles/260
