Faculty Articles
Flow Cytometric Isolation of Differentiated Cell-types from Embryoid Bodies
Publication Title
University of California
Publication Date
6-1-1993
Abstract
Induced differentiation of pluripotent embryonal carcinoma stem cells has been characterized as a cell culture model of early mammalian development. Since these cells can be prepared in bulk, they offer the opportunity to isolate relatively large amounts of biological material analogous to cell-types in the developing embryo; cell types that are both few in number and difficult to identify. We have established and morphologically and molecularly characterized differentiated embryoid bodies derived from the embryonal carcinoma cell line F9. Using the technique of fluorescent-activated cell sorting we were able to distinguish and isolate two differentiated cell-types from this model system based upon differences in their autofluorescence. We were then able to molecularly characterize expression of the endodermal marker alpha-fetoprotein in these isolated cell populations.
Disciplines
Medical Specialties | Medicine and Health Sciences | Osteopathic Medicine and Osteopathy
NSUWorks Citation
Pedersen, Roger A.; Grant, Stephen G.; Burdsal, Carol A.; and Latimer, Jean Johanna, "Flow Cytometric Isolation of Differentiated Cell-types from Embryoid Bodies" (1993). Faculty Articles. 887.
https://nsuworks.nova.edu/hpd_com_faculty_articles/887
