Comparison of Established Cell Lines at Different Passages by Karyotype and Comparative Genomic Hybridization

Authors

Sharon L. Wenger
Jamie R. Senft
Linda M. Sargent
Narendra Bairwa
Stephen G. Grant, Nova Southeastern UniversityFollow

Publication Title

Bioscience Reports

ISSN

0144-8463

Publication Date

12-1-2004

Abstract

Two established cancer cell lines, MCF-7 and Ishikawa, were both obtained directly from a cell repository and through another laboratory. The karyotypes from the two MCF-7 cell lines had up to 83 chromosomes and similarities for chromosomal gain and structural abnormalities. The two Ishikawa cell lines had up to 60 chromosomes with only a missing X as the common chromosome abnormality. CGH studies were performed by co-hybridizing the two Ishikawa or MCF-7 cell lines to normal metaphases. The differences seen between the two MCF-7 cell cultures reflect changes due to passage number and culture conditions. For Ishikawa, DNA polymorphic data and mutation studies suggest that the two cell lines are not derived from the same established tumor cell line. Our study shows the utilization of CGH in comparing cell lines originating from the same specimen. Our study also demonstrates the necessity for periodically evaluating cell lines to confirm their origin.

DOI

10.1007/s10540-005-2797-5

Volume

12

Issue

6

Disciplines

Medical Specialties | Medicine and Health Sciences | Osteopathic Medicine and Osteopathy

NSUWorks Citation

Wenger, Sharon L.; Senft, Jamie R.; Sargent, Linda M.; Bairwa, Narendra; and Grant, Stephen G., "Comparison of Established Cell Lines at Different Passages by Karyotype and Comparative Genomic Hybridization" (2004). Faculty Articles. 721.
https://nsuworks.nova.edu/hpd_com_faculty_articles/721