Faculty Articles
Title
Comparison of Established Cell Lines at Different Passages by Karyotype and Comparative Genomic Hybridization
ISBN or ISSN
0144-8463
Publication Title
Bioscience Reports
Volume
12
Issue
6
Publication Date / Copyright Date
12-1-2004
DOI Number
10.1007/s10540-005-2797-5
Abstract
Two established cancer cell lines, MCF-7 and Ishikawa, were both obtained directly from a cell repository and through another laboratory. The karyotypes from the two MCF-7 cell lines had up to 83 chromosomes and similarities for chromosomal gain and structural abnormalities. The two Ishikawa cell lines had up to 60 chromosomes with only a missing X as the common chromosome abnormality. CGH studies were performed by co-hybridizing the two Ishikawa or MCF-7 cell lines to normal metaphases. The differences seen between the two MCF-7 cell cultures reflect changes due to passage number and culture conditions. For Ishikawa, DNA polymorphic data and mutation studies suggest that the two cell lines are not derived from the same established tumor cell line. Our study shows the utilization of CGH in comparing cell lines originating from the same specimen. Our study also demonstrates the necessity for periodically evaluating cell lines to confirm their origin.
Disciplines
Medical Specialties | Medicine and Health Sciences | Osteopathic Medicine and Osteopathy
NSUWorks Citation
Wenger, Sharon L.; Senft, Jamie R.; Sargent, Linda M.; Bairwa, Narendra; and Grant, Stephen G., "Comparison of Established Cell Lines at Different Passages by Karyotype and Comparative Genomic Hybridization" (2004). Faculty Articles. 721.
https://nsuworks.nova.edu/hpd_com_faculty_articles/721