Faculty Articles
Renal expression of the gene encoding the gastric H(+)-K(+)-ATPase beta-subunit.
Publication Title
The American journal of physiology
Publisher
American Physiological Society
ISSN
0002-9513
Publication Date
3-1-1995
Keywords
Animals, Antibodies, Monoclonal, Base Sequence, DNA, Complementary, Gastric Mucosa, Gene Expression, Genes, Regulator, H(+)-K(+)-Exchanging ATPase, Immunohistochemistry, Kidney, Mice, Mice, Transgenic, Molecular Sequence Data, Polymerase Chain Reaction, Protein Conformation, RNA, Messenger, Rabbits, Swine
Abstract
The gastric mucosal parietal cells and cells of the renal collecting duct both possess H(+)-K(+)-adenosinetriphosphatase (H(+)-K(+)-ATPase) activities. In the stomach, the H(+)-K(+)-ATPase (EC 3.6.1.3) is responsible for acidification of luminal contents. The kidney H(+)-K(+)-ATPase protein(s) contribute to potassium reabsorption and secretion of hydrogen ions to maintain potassium and acid-base homeostasis. The stomach H(+)-K(+)-ATPase is well defined and consists of an alpha-catalytic subunit of apparent molecular mass of 95 kDa and a highly glycosylated beta-subunit of 60-90 kDa. The molecular identity of the protein that mediates the H(+)-K(+)-ATPase activity in the kidney has been addressed in this paper. A combination of RNA hybridizations, polymerase chain reaction analysis of kidney RNA, and sequence analysis of cDNAs indicated that gastric H(+)-K(+)-ATPase beta-subunit mRNA is present in kidney. Immunoblotting with antibodies specific for the gastric H(+)-K(+)-ATPase beta-subunit detected proteins, which, after deglycosylation, had the same molecular mass as the gastric beta-subunit in membrane protein preparations from rabbit, pig, rat, and mouse kidneys. Furthermore, we have used transgenic mice to demonstrate that the gastric H(+)-K(+)-ATPase beta-subunit gene contains cis-acting regulatory sequences that are active in both gastric parietal cells and the renal collecting ducts. Overall, these data indicate that the gastric H(+)-K(+)-ATPase beta-subunit is found in the kidney and probably associates with the gastric H(+)-K(+)-ATPase alpha-subunit and/or other P-type ATPase alpha-subunits, thus contributing to acid-base and potassium homeostasis.
DOI
10.1152/ajprenal.1995.268.3.F363
Volume
268
Issue
3 Pt 2
First Page
363
Last Page
374
Disciplines
Medical Specialties | Medicine and Health Sciences | Osteopathic Medicine and Osteopathy
NSUWorks Citation
Callaghan, J M; Tan, S S; Khan, M A; Curran, K A; Campbell, W Grady; Smolka, A J; Toh, B H; Gleeson, P A; Wingo, C S; Cain, B D; and Van Driel, I R., "Renal expression of the gene encoding the gastric H(+)-K(+)-ATPase beta-subunit." (1995). Faculty Articles. 1542.
https://nsuworks.nova.edu/hpd_com_faculty_articles/1542
