Chemistry and Physics Faculty Proceedings, Presentations, Speeches, Lectures


A P450 Metabolism Experiment for Undergraduate Biochemistry Laboratories

Event Name/Location

Division of Chemical Education ACS national meeting in Dallas, TX March 2014

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A laboratory experiment is described to provide students hands-on experience in learning some aspects of microsomal P450-catalyzed metabolism. Undergraduate students in the biochemistry laboratories detect and quantify the metabolites produced from butylated hydroxytoluene by liver microsomes using Gas Chromatography-Mass Spectrometry (GC-MS) techniques. The laboratory provides training to students for handling active microsomes, sample cleanup of biological matrices, extraction of organic metabolites, GC-MS analysis and data interpretation of complex mixtures, and collaborative work.