Chemistry and Physics Faculty Proceedings, Presentations, Speeches, Lectures
A P450 Metabolism Experiment for Undergraduate Biochemistry Laboratories
Event Name/Location
Division of Chemical Education ACS national meeting in Dallas, TX March 2014
Presentation Date
3-17-2014
Document Type
Poster
Description
A laboratory experiment is described to provide students hands-on experience in learning some aspects of microsomal P450-catalyzed metabolism. Undergraduate students in the biochemistry laboratories detect and quantify the metabolites produced from butylated hydroxytoluene by liver microsomes using Gas Chromatography-Mass Spectrometry (GC-MS) techniques. The laboratory provides training to students for handling active microsomes, sample cleanup of biological matrices, extraction of organic metabolites, GC-MS analysis and data interpretation of complex mixtures, and collaborative work.
NSUWorks Citation
Chiafair, Catherine; Giarikos, Dimitri; and Razeghifard, Reza, "A P450 Metabolism Experiment for Undergraduate Biochemistry Laboratories" (2014). Chemistry and Physics Faculty Proceedings, Presentations, Speeches, Lectures. 1.
https://nsuworks.nova.edu/cnso_chemphys_facpres/1
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