Chemistry and Physics Faculty Articles
Title
Modulation of Tumor Progression by Glycosylated Triple-helical Ligands
Document Type
Article
Publication Date
3-2-2006
Publication Title
FASEB Journal
ISSN
0892-6638
Volume
20
Issue/No.
4
First Page
A515
Abstract
Specific tumor cell interactions with basement membrane (type IV) or fibrillar (types I–III) collagen have been shown previously to represent a regulatory step in metastasis [Nat. Rev. Cancer3, 422 (2003)]. Interestingly, numerous tumor cell receptors, such as integrins, CD44 proteoglycan, and DDR Tyr kinases, bind sites within distinct triple-helical regions of collagen that are glycosylated. The present study has refined methodology for constructing galactosylated hydroxylysine [Hyl(O-ß-D-galactopyranosyl)]-containing triple-helical ligands, and utilized these ligands to evaluate binding and activation of (a) 2ß 1 and 3ß 1 integrins by 1(IV)382–393 and 1(V)531–543, respectively, (b) CD44/CSPG by 1(IV)1263–1277, and (c) DDR2 by 1(I)79–93. The possible effect of glycosylation on ligand conformation, and hence the correlation of structure with activity, has been studied by CD spectroscopy. Initial results showed that glycosylation significantly decreased CD44-mediated adhesion and spreading of melanoma cells [J. Biol. Chem. 278, 14321 (2003)]. This was the first demonstration of the prophylactic effects of glycosylation on tumor cell interaction with the basement membrane, and suggested a possible cryptic sites mechanism associated with tumor cell invasion.
NSUWorks Citation
Fields, G. B., Cudic, M., Aukszi, B., & Lauer-Fields, J. (2006). Modulation of Tumor Progression by Glycosylated Triple-helical Ligands. FASEB Journal, 20, (4), A515 - . Retrieved from https://nsuworks.nova.edu/cnso_chemphys_facarticles/5
