Chemistry and Physics Faculty Articles
Title
Applications of Cryostructures in the Chromatographic Separation of Biomacromolecules
Document Type
Article
Publication Date
9-30-2022
Publication Title
Journal of Chromatography A
Keywords
Cryostructures, Cryogels, Chromatography, Stationary phase, Biomacromolecules
ISSN
1873-3778
Volume
1683
First Page
463546
Abstract
Macroporous-interconnected cryostructures are prepared via cryoprocess approaches, where polymerization, crosslinking-polymerization, or even chain aggregation is primarily conducted at the sub-freezing temperature of the medium followed by thawing. Given their unique desirable properties, cryostructures have been studied as a stationary phase in chromatography to purify and separate proteins. The macroporous cryostructures with their highly interconnected large (10–100 µm) pores have various advantages, including the high surface area, sponge-like morphology, high elasticity, low-pressure drop, short diffusion path, and a temporary residence. In this review, we elaborate on the methods used in the preparation of cryostructures and the factors affecting their properties. We also highlight the underlying progress in chromatography techniques that take advantage of cryostructures as stationary phases for separating multiple biomacromolecules.
NSUWorks Citation
Babanejad, N., Mfoafo, K. A., Zhang, E., Omidi, Y., Razeghifard, R., & Omidian, H. (2022). Applications of Cryostructures in the Chromatographic Separation of Biomacromolecules. Journal of Chromatography A, 1683, 463546 - . https://doi.org/10.1016/j.chroma.2022.463546. Retrieved from https://nsuworks.nova.edu/cnso_chemphys_facarticles/307
ORCID ID
https://orcid.org/0000-0001-5054-4338, https://orcid.org/0000-0002-5637-8729
DOI
10.1016/j.chroma.2022.463546
Comments
0021-9673/© 2022 Elsevier B.V. All rights reserved.
The authors would like to thank Nova Southeastern University’s Health Professions Division for funding this research.