Chemistry and Physics Faculty Articles

Applications of Cryostructures in the Chromatographic Separation of Biomacromolecules

ORCID

https://orcid.org/0000-0001-5054-4338, https://orcid.org/0000-0002-5637-8729

Document Type

Article

Publication Title

Journal of Chromatography A

ISSN

1873-3778

Publication Date

9-30-2022

Keywords

Cryostructures, Cryogels, Chromatography, Stationary phase, Biomacromolecules

Abstract

Macroporous-interconnected cryostructures are prepared via cryoprocess approaches, where polymerization, crosslinking-polymerization, or even chain aggregation is primarily conducted at the sub-freezing temperature of the medium followed by thawing. Given their unique desirable properties, cryostructures have been studied as a stationary phase in chromatography to purify and separate proteins. The macroporous cryostructures with their highly interconnected large (10–100 µm) pores have various advantages, including the high surface area, sponge-like morphology, high elasticity, low-pressure drop, short diffusion path, and a temporary residence. In this review, we elaborate on the methods used in the preparation of cryostructures and the factors affecting their properties. We also highlight the underlying progress in chromatography techniques that take advantage of cryostructures as stationary phases for separating multiple biomacromolecules.

DOI

10.1016/j.chroma.2022.463546

Volume

1683

First Page

463546

Comments

0021-9673/© 2022 Elsevier B.V. All rights reserved.

The authors would like to thank Nova Southeastern University’s Health Professions Division for funding this research.

This document is currently not available here.

Peer Reviewed

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