NMR-Based Molecular Ruler for Determining the Depth of Intercalants Within the Lipid Bilayer. Part V: A Comparison of Liposomes, Bioliposomes and Erythrocyte Ghosts
Chemistry and Physics of Lipids
DMPC liposome, Bioliposome, Erythrocyte ghost, 13C NMR, ET(30), Ketophospholipids
Afri et al., 2014a, Afri et al., 2014b have recently reported their mapping of DMPC liposomes using 13C NMR in conjunction with a wide range of difunctional intercalants: n-ketoesters, n-ketoacids and n-ketophosphatidylcholines. The present study initiates a comparable study of bioliposomes and erythrocyte ghosts. This required the 13C NMR characterization of these systems for the first time, and further involved a determination of the signals of three doubly 13C-labeled intercalants, in particular, n-ketophosphatidylcholines where n = 4, 8 and 12. This study reveals that DMPC liposomes, bioliposomes and erythrocyte ghosts, with all their structural differences, are not radically different from the perspective of polarity gradient. Any differences observed reflect the additives often naturally present in these lipid systems.
Afri, M., Alexenberg, C., Bodner, E., Frimer, A. A., Jacob, A. I., & Naqqash, M. E. (2014). NMR-Based Molecular Ruler for Determining the Depth of Intercalants Within the Lipid Bilayer. Part V: A Comparison of Liposomes, Bioliposomes and Erythrocyte Ghosts. Chemistry and Physics of Lipids, 184, 52 - 60. https://doi.org/10.1016/j.chemphyslip.2014.10.002. Retrieved from https://nsuworks.nova.edu/cnso_chemphys_facarticles/272