Chemistry and Physics Faculty Articles
Title
NMR-Based Molecular Ruler for Determining the Depth of Intercalants Within the Lipid Bilayer. Part V: A Comparison of Liposomes, Bioliposomes and Erythrocyte Ghosts
Document Type
Article
Publication Date
12-2014
Publication Title
Chemistry and Physics of Lipids
Keywords
DMPC liposome, Bioliposome, Erythrocyte ghost, 13C NMR, ET(30), Ketophospholipids
ISSN
0009-3084
Volume
184
First Page
52
Last Page
60
Abstract
Afri et al., 2014a, Afri et al., 2014b have recently reported their mapping of DMPC liposomes using 13C NMR in conjunction with a wide range of difunctional intercalants: n-ketoesters, n-ketoacids and n-ketophosphatidylcholines. The present study initiates a comparable study of bioliposomes and erythrocyte ghosts. This required the 13C NMR characterization of these systems for the first time, and further involved a determination of the signals of three doubly 13C-labeled intercalants, in particular, n-ketophosphatidylcholines where n = 4, 8 and 12. This study reveals that DMPC liposomes, bioliposomes and erythrocyte ghosts, with all their structural differences, are not radically different from the perspective of polarity gradient. Any differences observed reflect the additives often naturally present in these lipid systems.
Additional Comments
Israel Science Foundation grant #s: 327/02, 437/06
NSUWorks Citation
Afri, M., Alexenberg, C., Bodner, E., Frimer, A. A., Jacob, A. I., & Naqqash, M. E. (2014). NMR-Based Molecular Ruler for Determining the Depth of Intercalants Within the Lipid Bilayer. Part V: A Comparison of Liposomes, Bioliposomes and Erythrocyte Ghosts. Chemistry and Physics of Lipids, 184, 52 - 60. https://doi.org/10.1016/j.chemphyslip.2014.10.002. Retrieved from https://nsuworks.nova.edu/cnso_chemphys_facarticles/272
DOI
10.1016/j.chemphyslip.2014.10.002
Comments
©2014 Elsevier Ireland Ltd. All rights reserved.