Chemistry and Physics Faculty Articles

Title

NMR-Based Molecular Ruler for Determining the Depth of Intercalants Within the Lipid Bilayer: Part III: Studies on Keto Esters and Acids

Document Type

Article

Publication Date

12-2014

Publication Title

Chemistry and Physics of Lipids

Keywords

Liposome, 13C NMR, ET, DMPC, Oxooctadecanoates, Molecular ruler

ISSN

0009-3084

Volume

184

First Page

105

Last Page

118

Abstract

The development of “molecular rulers” would allow one to quantitatively locate the penetration depth of intercalants within lipid bilayers. To this end, an attempt was made to correlate the 13C NMR chemical shift of polarizable “reporter” carbons (e.g., carbonyls) of intercalants within DMPC liposomal bilayers – with the polarity it experiences, and with its Angstrom distance from the interface. This requires families of molecules with two “reporter carbons” separated by a known distance, residing at various depths/polarities within the bilayer. For this purpose, two homologous series of dicarbonyl compounds, methyl n-oxooctadecanoates and the corresponding n-oxooctadecanoic acids (n = 4–16), were synthesized. To assist in assignment and detection several homologs in each system were prepared 13C-enriched in both carbonyls. Within each family, the number of carbons and functional groups remains the same, with the only difference being the location of the second ketone carbonyl along the fatty acid chain. Surprisingly, the head groups within each family are not anchored near the lipid–water interface, nor are they even all located at the same depth. Nevertheless, using an iterative best fit analysis of the data points enables one to obtain an exponential curve. The latter gives substantial insight into the correlation between polarity (measured in terms of the Reichardt polarity parameter, ET(30)) and penetration depth into the liposomal bilayer. Still missing from this curve are data points in the moderate polarity range.

Comments

©2014 Elsevier Ireland Ltd. All rights reserved.

Additional Comments

Israel Science Foundation grant #s: 327/02, 437/06

DOI

10.1016/j.chemphyslip.2014.07.007

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