Chemistry and Physics Faculty Articles
Characterization of MYC-Induced Tumorigenesis by in Situ Lipid Profiling
Document Type
Article
Publication Title
Analytical Chemistry
ISSN
0003-2700
Publication Date
5-7-2013
Abstract
We apply desorption electrospray ionization mass spectrometry imaging (DESI-MSI) to provide an in situ lipidomic profile of genetically modified tissues from a conditional transgenic mouse model of MYC-induced hepatocellular carcinoma (HCC). This unique, label-free approach of combining DESI-MSI with the ability to turn specific genes on and off has led to the discovery of highly specific lipid molecules associated with MYC-induced tumor onset. We are able to distinguish normal from MYC-induced malignant cells. Our approach provides a strategy to define a precise molecular picture at a resolution of about 200 μm that may be useful in identifying lipid molecules that define how the MYC oncogene initiates and maintains tumorigenesis.
DOI
10.1021/ac400479j
Volume
85
Issue
9
First Page
4529
Last Page
4262
Additional Comments
Air Force Office of Scientific Research grant #: FA 9550-10-1-0235; NIH RO1 grant #: CA 089305, 105102; National Cancer Institute's In-vivo Cellular and Molecular Imaging Center grant #: CA 114747; Integrative Cancer Biology Program grant #: CA 112973; NIH/NCI PO1 grant #: CA034233; Leukemia and Lymphoma Society Translational Research grant #: R6223-07
NSUWorks Citation
Perry, R. H., Bellovin, D. I., Shroff, E. H., Ismail, A. I., Zubuawala, T., Felsher, D. W., & Zare, R. N. (2013). Characterization of MYC-Induced Tumorigenesis by in Situ Lipid Profiling. Analytical Chemistry, 85, (9), 4529 - 4262. https://doi.org/10.1021/ac400479j. Retrieved from https://nsuworks.nova.edu/cnso_chemphys_facarticles/190
Comments
©2013 American Chemical Society