Title

MicroRNA‐132 Directs Human Periodontal Ligament‐Derived Neural Crest Stem Cell Neural Differentiation

Authors

Tsz Kin Ng, Shantou University - China; The Chinese University of Hong Kong
Qichen Yang, The Chinese University of Hong Kong
Veronica R. Fortino, University of Miami; Nova Southeastern University
Nikky Yuk-Ki Lai, The Chinese University of Hong Kong
Carlos M. Carballosa, University of Miami
Jordan M. Greenberg, University of Miami
Kwong Wai Choy, The Chinese University of Hong Kong
Daniel Pelaez, Miami Veterans Affairs Medical Center; University of Miami
Chi Pui Pang, Shantou University - China; The Chinese University of Hong Kong
Herman S. Cheung, Miami Veterans Affairs Medical Center; University of Miami

Document Type

Article

Publication Date

10-23-2018

Publication Title

Journal of Tissue Engineering and Regenerative Medicine

Keywords

Neural crest stem cells, Periodontal ligament, Neural differentiation, MicroRNAs, ZEB2, Neurons

ISSN

1932-6254

First Page

1

Last Page

31

Abstract

Neurogenesis is the basis of stem cell tissue engineering and regenerative medicine for central nervous system (CNS) disorders. We have established differentiation protocols to direct human periodontal ligament‐derived stem cells (PDLSCs) into neuronal lineage, and we recently isolated the neural crest sub‐population from PDLSCs, which are pluripotent in nature. Here we report the neural differentiation potential of these periodontal ligament‐derived neural crest stem cells (NCSCs) as well as its microRNA (miRNA) regulatory mechanism and function in NCSC neural differentiation. NCSCs, treated with basic fibroblast growth factor and epidermal growth factor‐based differentiation medium for 24 days, expressed neuronal and glial markers (βIII‐tubulin, neurofilament, NeuN, neuron‐specific enolase, GFAP and S100) and exhibited glutamate‐induced calcium responses. The global miRNA expression profiling identified 60 upregulated and 19 downregulated human miRNAs after neural differentiation, and the gene ontology analysis of the miRNA target genes confirmed the neuronal differentiation‐related biological functions. In addition, overexpression of miR‐132 in NCSCs promoted the expression of neuronal markers and downregulated ZEB2 protein expression. Our results suggested that the pluripotent NCSCs from human periodontal ligament can be directed into neural lineage, which demonstrate its potential in tissue engineering and regenerative medicine for CNS disorders.

Comments

©2018 John Wiley & Sons, Inc. All rights reserved

NSUWorks Citation

Kin Ng, Tsz; Qichen Yang; Veronica R. Fortino; Nikky Yuk-Ki Lai; Carlos M. Carballosa; Jordan M. Greenberg; Kwong Wai Choy; Daniel Pelaez; Chi Pui Pang; and Herman S. Cheung. 2018. "MicroRNA‐132 Directs Human Periodontal Ligament‐Derived Neural Crest Stem Cell Neural Differentiation." Journal of Tissue Engineering and Regenerative Medicine , (): 1-31. doi:10.1002/term.2759.

DOI

10.1002/term.2759