Biology Faculty Articles

Document Type

Article

Publication Date

6-2018

Publication Title

Advanced Science

Keywords

Bioinspired materials, Differential extraction, DNA casework backlog, Forensic cases, Microfluids

ISSN

2198-3844

Volume

2018

Issue/No.

1800121

First Page

1

Last Page

12

Abstract

One out of every six American women has been the victim of a sexual assault in their lifetime. However, the DNA casework backlog continues to increase outpacing the nation's capacity since DNA evidence processing in sexual assault casework remains a bottleneck due to laborious and time‐consuming differential extraction of victim's and perpetrator's cells. Additionally, a significant amount (60–90%) of male DNA evidence may be lost with existing procedures. Here, a microfluidic method is developed that selectively captures sperm using a unique oligosaccharide sequence (Sialyl‐LewisX), a major carbohydrate ligand for sperm‐egg binding. This method is validated with forensic mock samples dating back to 2003, resulting in 70–92% sperm capture efficiency and a 60–92% reduction in epithelial fraction. Captured sperm are then lysed on‐chip and sperm DNA is isolated. This method reduces assay‐time from 8 h to 80 min, providing an inexpensive alternative to current differential extraction techniques, accelerating identification of suspects and advancing public safety.

Comments

© 2018 The Authors. Published by WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

advs670-sup-0001-s1.pdf (1181 kB)
Supplementary File 1

advs670-sup-0001-s1.mp4 (4166 kB)
Supplementary File 2

advs670-sup-0002-s2.mp4 (5188 kB)
Supplementary File 3

ORCID ID

0000-0002-4931-5316

DOI

10.1002/advs.201800121

Peer Reviewed

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