HSV-2-induced Tumorigenicity in HPV 16-immortalized Human Genital Keratinocytes

Authors

• Joseph A. Dipaolo, National Cancer Institute - Bethesda
• Craig D. Woodworth, National Cancer Institute - Bethesda
• Nicholas C. Popescu, National Cancer Institute - Bethesda
• Debra L. Koval, National Cancer Institute - Bethesda
• Jose V. Lopez, National Cancer Institute - BethesdaFollow
• Jay Doniger, National Cancer Institute - Bethesda

ORCID

0000-0002-1637-4125

ResearcherID

F-8809-2011

Document Type

Article

Publication Title

Virology

ISSN

0042-6822

Publication Date

8-1990

Keywords

Carcinoma, Squamous cell/microbiology, Carcinoma squamous cell/pathology, Cell transformation neoplastic, Cells cultured, DNA viral/genetics, Epithelial cells, Female, Genes viral, Humans, Keratinocytes/cytology, Papillomaviridae/genetics, Simplexvirus/genetics, Transfection, Uterine cervical neoplasms/microbiology, Uterine cervical neoplasms/pathology

Abstract

A subgenomic region of HSV-2, BglII N, is capable of converting immortal genital epithelial cells containing integrated HPV16 sequences into tumorigenic squamous cell carcinoma cells. Moreover, tumor-derived cultured cells and immortal cells that had been transfected with HSV-2/BglII N and kept in continuous culture subsequently lost the HSV-2 sequences. The HSV-2/Bg/II N sequence was ineffective on normal cells. Thus, HSV-2/Bg/II N may act as a cofactor in the genesis of a carcinoma but is not required to maintain the transformed phenotype. Although pa pillornaviruses (HPVs) are currently receiving much attention because of their association with cervical squamous carcinomas, ample reasons exist to suggest a multifactorial etiology in which additional factors are necessary to convert dysplastic lesions to carcinomas. The hypothesis that specific HPV types may be necessary but not sufficient to cause cancer is reinforced. Thus, HSV-2-transforming sequences have a potential role in the etiology of human cervical cancer.

DOI

10.1016/0042-6822(90)90548-6

Volume

177

Issue

2

First Page

777

Last Page

779

Comments

©1990 Elsevier Inc.

NSUWorks Citation

Dipaolo, Joseph A.; Craig D. Woodworth; Nicholas C. Popescu; Debra L. Koval; Jose V. Lopez; and Jay Doniger. 1990. "HSV-2-induced Tumorigenicity in HPV 16-immortalized Human Genital Keratinocytes." Virology 177, (2): 777-779. doi:10.1016/0042-6822(90)90548-6.