Title

Characterization of Genetic Markers for In Vitro Cell Line Identification of the Marine Sponge Axinella corrugata

Authors

Jose V. Lopez, Harbor Branch Oceanographic InstitutionFollow
C. L. Peterson, Harbor Branch Oceanographic Institution
Robin Willoughby, Harbor Branch Oceanographic Institution
A. E. Wright, Harbor Branch Oceanographic Institution
E. Enright, Harbor Branch Oceanographic Institution
S. Zoladz, Harbor Branch Oceanographic Institution
John K. Reed, Harbor Branch Oceanographic Institution
Shirley A. Pomponi, Harbor Branch Oceanographic Institution

Document Type

Article

Publication Date

1-1-2002

Publication Title

Journal of Heredity

Keywords

Base sequence, Cell line, DNA fingerprinting, Genetic markers, Molecular sequence data, Phylogeny, Porifera, Sequence alignment, Sequence analysis

ISSN

0022-1503

Volume

93

Issue/No.

1

First Page

27

Last Page

36

Abstract

The marine sponge Axinella corrugata is being developed as a model organism for in vitro marine invertebrate research. Molecular genetics methods such as DNA fingerprinting [amplified fragment length polymorphism (AFLP) and single-stranded conformation polymorphism (SSCP)] and single-locus DNA sequence analyses were applied to this model to meet the primary objective of identifying positive A. corrugata–specific molecular markers that will aid in verifying cell identity in vitro and distinguish sponge cells from potential microbial contaminants. The extent of intra- and interspecific variation in these markers from geographically distinct samples of A. corrugata and closely related sponge taxa was also assessed. Two novel nuclear loci along with intervening transcribed spacer (ITS) regions of nuclear rRNA were characterized, although the latter appeared to better meet primary marker criteria, such as taxonomic specificity and high frequency of detection (via polymerase chain reaction [PCR]) from different individuals (n > 40) and cell cultures. Phylogenetic and phylogeographic analyses of ITS DNA sequences helped clarify taxonomies and also suggested species boundaries between and among western Atlantic and eastern Atlantic/Indian Ocean A. corrugataand Axinellidae samples. Patterns of genetic variation have important implications for the systematics, evolution, and chemical ecology of A. corrugata and related axinellids and are discussed.

Comments

©2002 The American Genetic Association

Additional Comments

Florida Sea Grant grant #: R/LR-MB-5

NSUWorks Citation

Lopez, Jose V.; C. L. Peterson; Robin Willoughby; A. E. Wright; E. Enright; S. Zoladz; John K. Reed; and Shirley A. Pomponi. 2002. "Characterization of Genetic Markers for In Vitro Cell Line Identification of the Marine Sponge Axinella corrugata." Journal of Heredity 93, (1): 27-36. doi:10.1093/jhered/93.1.27.

ORCID ID

0000-0002-1637-4125

ResearcherID

F-8809-2011

DOI

10.1093/jhered/93.1.27