Biology Faculty Articles
Development of MHC-Linked Microsatellite Markers in the Domestic Cat and Their Use to Evaluate MHC Diversity in Domestic Cats, Cheetahs, and Gir Lions
Journal of Heredity
Acinonyx jubatus, Felis catus, Major histocompatibility complex, Panthera leo
Diversity within the major histocompatibility complex (MHC) reflects the immunological fitness of a population. MHC-linked microsatellite markers provide a simple and an inexpensive method for studying MHC diversity in large-scale studies. We have developed 6 MHC-linked microsatellite markers in the domestic cat and used these, in conjunction with 5 neutral microsatellites, to assess MHC diversity in domestic mixed breed (n = 129) and purebred Burmese (n = 61) cat populations in Australia. The MHC of outbred Australian cats is polymorphic (average allelic richness = 8.52), whereas the Burmese population has significantly lower MHC diversity (average allelic richness = 6.81; P < 0.01). The MHC-linked microsatellites along with MHC cloning and sequencing demonstrated moderate MHC diversity in cheetahs (n = 13) and extremely low diversity in Gir lions (n = 13). Our MHC-linked microsatellite markers have potential future use in diversity and disease studies in other populations and breeds of cats as well as in wild felid species.
National Cancer Institute contract #: N01-CO-12400; Russian Ministry of Science grant #: 11.G34.31.0068
Morris, Katrina M.; Katherine Kirby; Julia A. Beatty; Vanessa R. Barrs; Sonia Cattley; Victor David; Stephen J. O'Brien; Marilyn Menotti-Raymond; and Katherine Belov. 2014. "Development of MHC-Linked Microsatellite Markers in the Domestic Cat and Their Use to Evaluate MHC Diversity in Domestic Cats, Cheetahs, and Gir Lions." Journal of Heredity 105, (4): 493-505. doi:10.1093/jhered/esu017.
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