Development and Characterization of 10 Polymorphic Microsatellite Loci for the Blue Shark, Prionace glauca, and Their Cross Shark-Species Amplification

Authors

• Sean Fitzpatrick, Queen's University - Belfast, United Kingdom
• Mahmood S. Shivji, Nova Southeastern UniversityFollow
• Demian D. Chapman, State University of New York - Stony Brook
• Paulo A. Prodohl, Queen's University - Belfast, United Kingdom

ResearcherID

G-4080-2013

Document Type

Article

Publication Title

Conservation Genetics Resources

ISSN

1877-7252

Publication Date

7-2011

Keywords

Blue shark, Prionace glauca, Carcharhinus, Polymorphic, Microsatellite, Cross-species

Abstract

Ten polymorphic nuclear microsatellite loci were developed from a microsatellite enriched genomic library of the blue shark, Prionace glauca. The utility of these markers for genetic studies of this globally distributed, heavily exploited, oceanic predator was assessed by screening 120 specimens sampled from six locations throughout the species’ range. Both moderately and highly polymorphic marker loci were identified. Three to 35 alleles were found to be segregating per locus (mean 10.1) with observed heterozygosities ranging from 24 to 91%. Evaluation of the cross-species amplification of these markers across 18 additional shark species indicates that these microsatellites are potentially useful for genetic studies of other species of conservation concern.

DOI

10.1007/s12686-011-9395-6

Volume

3

Issue

3

First Page

523

Last Page

527

Comments

©Springer Science+Business Media B.V. 2011

NSUWorks Citation

Fitzpatrick, Sean; Mahmood S. Shivji; Demian D. Chapman; and Paulo A. Prodohl. 2011. "Development and Characterization of 10 Polymorphic Microsatellite Loci for the Blue Shark, Prionace glauca, and Their Cross Shark-Species Amplification." Conservation Genetics Resources 3, (3): 523-527. doi:10.1007/s12686-011-9395-6.